加工马铃薯Atlantic无标记基因遗传转化体系建立

发布时间：2018-03-25 06:47

  本文选题：Atlantic　切入点：无标记基因　出处：《分子植物育种》2017年11期

【摘要】：本研究以加工型专用品种Atlantic为实验材料,分别对影响其遗传转化的外植体、激素、预培养、菌液浓度、侵染及共培养等因素进行优化,建立了农杆菌介导的无标记基因高效转化体系,初步获得了转基因植株。研究结果表明,叶片和茎段均可作为马铃薯品种Atlantic的受体材料,转化过程中以预培养2~3 d后用OD600=0.5的菌液侵染10~15 min,在MSGⅠ(添加有5 mg/L NAA和1.0 mg/L 6-BA)培养基上共培养2~3d后,转入含有250 mg/L的MSGⅡ(0.02 mg/L GA3和2.0 g/L ZT)的培养基上诱导生芽的遗传转化效率最高,其愈伤诱导率可达83.34%,芽分化率可达48.3%。通过该转化体系将抗马铃薯PVY病毒的基因导入马铃薯,获得无标记基因的转化植株,经PCR检测,初步确定已经将外源目的基因导入了马铃薯基因组中。
[Abstract]:In this study, the explant, hormone, preculture, bacterial liquid concentration, infection and co-culture were optimized with Atlantic as the experimental material. Agrobacterium tumefaciens mediated high efficiency transformation system of unlabeled genes was established and transgenic plants were obtained. The results showed that both leaf and stem segments could be used as receptor materials for potato variety Atlantic. During the transformation, the cells were precultured for 2 days, then infected with OD600=0.5 solution for 1015 min, then co-cultured on the medium of MSG 鈪，

本文编号：1662008