珠江上游地区蚊虫乙型脑炎病毒核酸检测及PrM和E基因序列分析

发布时间：2018-04-03 04:31

本文选题：乙型脑炎病毒　切入点：蚊虫分类　出处：《中国畜牧兽医》2017年02期

【摘要】：试验旨在了解珠江上游地区乙型脑炎病毒(JEV)主要传播媒介蚊虫的种类、分布及携带JEV基因型和分子特征。2013年7月在珠江上游地区师宗县采集蚊虫和库蠓标本,并对蚊虫进行分类鉴定;同时采用黄病毒属引物对采集到的蚊虫和库蠓标本进行检测,阳性标本用JEV PrM和E基因特异引物进行扩增、测序,然后采用生物信息学软件进行序列分析。在4个采集点共采集蚊虫3 705只,分别属于库蚊、按蚊、伊蚊和阿蚊4个属的7种蚊虫,牛圈以中华按蚊、骚扰阿蚊为优势蚊种,羊圈以中华按蚊、骚扰阿蚊为优势蚊种,猪圈以三带喙库蚊、中华按蚊和骚扰阿蚊为优势蚊种。采用JEV PrM和E基因特异引物对采集到蚊虫和库蠓标本分162批进行扩增、测序,采用生物信息学软件进行序列分析。结果显示,4株蚊虫携带的病毒均为基因Ⅰ型JEV,与疫苗株SA14-14-2E蛋白存在15个氨基酸差异位点,在8个影响病毒毒力的重要位点上未发生突变。以上结果提示珠江上游地区存在多种蚊虫种类,三带喙库蚊和中华按蚊是该地区的优势蚊种,三带喙库蚊是当地JEV的主要传播媒介,在当地的媒介中存在基因Ⅰ型JEV流行,而流行株的毒力并未降低。
[Abstract]:The aim of the experiment was to understand the species, distribution and JEV genotypes and molecular characteristics of Japanese encephalitis virus (JEV) in the upper reaches of the Pearl River. The specimens of mosquitoes and Culicomidges were collected in Shizong County, the upper reaches of the Pearl River in July 2013.At the same time, the samples of mosquitoes and Culicoides were detected by using yellow virus primer, and the positive samples were amplified and sequenced by JEV PrM and E gene specific primers.Then the sequence analysis was carried out with bioinformatics software.A total of 3 705 mosquitoes belonging to 4 genera of Culex pipiens, Anopheles aegypti and Aedes albopictus were collected in 4 collection sites. Anopheles sinensis was the dominant mosquito species in cattle circles, Anopheles sinensis as dominant species, Anopheles sinensis as dominant species and Anopheles albopictus as dominant species.Culex tritaeniorhynchus, Anopheles sinensis and Anopheles albopictus are the dominant species in pigsty.JEV PrM and E gene specific primers were used to amplify the samples of mosquitoes and Culicomidges in 162 batches, sequenced, and sequenced by bioinformatics software.The results showed that the viruses carried by the four strains were all gene type I, and there were 15 amino acid difference sites between them and the SA14-14-2E protein of the vaccine strain, and no mutation was found at the 8 important sites that affected the virulence of the virus.These results suggest that there are many mosquito species in the upper reaches of the Pearl River. Culex tritaeniorhynchus and Anopheles sinensis are the dominant species in this area. Culex tritaeniorhynchus is the main transmission vector of JEV in the region, and the gene type I JEV is prevalent in the local vectors.But the virulence of the epidemic strain did not decrease.
【作者单位】：云南省畜牧兽医科学院云南省热带亚热带动物病毒重点实验室;云南省昆明市东川区动物疫病预防控制中心;
【基金】：国家自然科学基金(31460669) 国家公益性(农业)行业专项(201303035)
【分类号】：R384.1;R373.31

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