咖啡碱和绿原酸对高脂饮食小鼠体质量、脂类沉积及肝脏脂质代谢基因表达的影响

发布时间：2018-04-06 02:15

本文选题：咖啡碱　切入点：绿原酸　出处：《食品科学》2017年09期

【摘要】：研究咖啡主要成分咖啡碱和绿原酸对高脂饮食诱导的肥胖小鼠体质量、脂类沉积及肝脏脂类代谢基因表达的影响。50只雌性ICR小鼠被随机分成5组:对照组、高脂组、咖啡碱、绿原酸、咖啡碱+绿原酸,给药组分别在饮水中添加0.05%咖啡碱、0.2%绿原酸、0.05%咖啡碱+0.2%绿原酸,饲养14周。饲养期间每周测1次体质量。饲养结束后心脏采血,摘取脏器和腹腔内脂肪(intraperitoneal adipose tissues,IPAT)并称质量。测定血糖浓度、血中及肝脏中脂质含量。通过实时定量聚合酶链式反应测定肝脏中脂质代谢相关基因AMPK、HMG-CoAr、FASN、ACO的mRNA表达量。与高脂组相比,咖啡碱+绿原酸能明显抑制小鼠体质量和IPAT质量的增加;咖啡碱、咖啡碱+绿原酸明显降低血糖和总胆固醇浓度;绿原酸投喂小鼠血中低密度脂蛋白胆固醇浓度显著降低,而血中游离脂肪酸浓度上升;咖啡碱、咖啡碱+绿原酸显著降低肝脏中总胆固醇和甘油三酯的含量;咖啡碱和咖啡碱+绿原酸使AMPK基因相对表达量显著上升,FASN基因相对表达量显著下降;3组给药组的ACO基因相对表达量显著上升,HMG-CoAr基因相对表达量显著下降。咖啡碱+绿原酸的减肥作用可能通过调节肝脏脂质代谢相关基因的表达,来降低血中和肝脏中脂类的含量,抑制脂肪沉积,抑制小鼠体质量增加,且它们有协同作用。
[Abstract]:To study the effects of caffeine and Lv Yuan on body mass, lipid deposition and liver lipid metabolism gene expression in obese mice induced by high fat diet. 50 female ICR mice were randomly divided into 5 groups: control group, high fat group, caffeine group.Lv Yuan acid, caffeine Lv Yuan acid, 0.05% caffeine 0.2wt% Lv Yuan acid were added to drinking water, respectively, and were fed for 14 weeks.Body mass was measured once a week during feeding.After feeding, the blood was taken from the heart, the viscera and intraperitoneal adipose tissues were taken and the quality was obtained.Blood glucose concentration and lipid content in blood and liver were measured.The expression of HMG-CoA FASNNCO-ACO in liver was determined by real-time quantitative polymerase chain reaction (RQPCR).Compared with hyperlipidemia group, theophylline Lv Yuan could significantly inhibit the increase of body mass and IPAT mass of mice, and caffeine and caffeine Lv Yuan acid could significantly decrease the concentration of blood glucose and total cholesterol.The concentration of serum low density lipoprotein cholesterol (LDL-C) and free fatty acid (FFA) increased significantly in mice fed with Lv Yuan, and caffeine and caffein Lv Yuan acid significantly decreased the contents of total cholesterol and triglyceride in liver.Theophylline and theophylline Lv Yuan caused a significant increase in the relative expression of AMPK gene. The relative expression of ACO gene in the three groups was significantly increased and the relative expression of HMG-CoAr gene was significantly decreased.Theophylline Lv Yuan may reduce the lipid content in blood and liver by regulating the expression of liver lipid metabolism related genes, inhibit fat deposition, inhibit the increase of body mass in mice, and they have synergistic effect.
【作者单位】：江西农业大学食品科学与工程学院江西省天然产物与功能性食品重点实验室;
【基金】：国家自然科学基金地区科学基金项目(31160320) 江西省自然科学基金项目(20142BAB204003)
【分类号】：TS201.4

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