缺氮对油菜养分吸收及钾吸收转运相关基因表达的影响

发布时间：2018-04-06 03:04

本文选题：甘蓝型油菜　切入点：缺氮　出处：《中国油料作物学报》2017年01期

【摘要】：为进一步了解缺氮对油菜养分吸收的影响,以甘蓝型油菜中双11号为材料,采用营养液培养方法,设置正常供氮和缺氮两个处理,培养14d后收获,分析缺氮对油菜植株表型、生物量、植株养分含量及养分吸收相关基因表达的影响。结果表明,缺氮严重抑制油菜植株生长,地上部生物量显著减少,叶片瘦小、黄化,茎秆呈紫红色。缺氮促进油菜根系生长,植株根冠比增加。缺氮处理对油菜叶、茎、根中大量元素含量的影响有所不同,缺氮油菜叶、茎、根的氮浓度及氮含量都显著降低;油菜叶、茎的磷浓度在缺氮胁迫时显著增加,根的磷浓度显著降低,而茎和根的磷含量在缺氮胁迫时都显著降低,但叶片磷含量不受影响;油菜不同部位钾浓度和钾含量在缺氮胁迫时都显著降低,并且缺氮处理14d显著抑制油菜大部分钾通道蛋白基因以及3个根部特异钾转运蛋白基因的表达,说明缺氮对油菜钾吸收运转相关基因表达的影响,可能导致油菜植株钾含量的降低。
[Abstract]:In order to further understand the effect of nitrogen deficiency on nutrient absorption of rapeseed, using Zhongshuang11 of Brassica napus as material, using nutrient solution culture method, two treatments of normal nitrogen supply and nitrogen deficiency were set up. After 14 days of cultivation, the effects of nitrogen deficiency on the phenotype of rape plant were analyzed.Effects of biomass, plant nutrient content and nutrient uptake related gene expression.The results showed that nitrogen deficiency seriously inhibited the growth of rape plants, significantly decreased the aboveground biomass, reduced the leaves, yellowed and purplish red stems.Nitrogen deficiency promoted root growth and root-shoot ratio of rape.The effects of nitrogen deficiency on the contents of a large number of elements in leaves, stems and roots of rape were different. The nitrogen concentration and content of nitrogen in leaves, stems and roots of nitrogen deficient rapeseed were significantly decreased, and the phosphorus concentration in stems of rape leaves increased significantly under nitrogen deficiency stress.P concentration in roots decreased significantly, but P content in stems and roots decreased significantly under nitrogen deficiency stress, but phosphorus content in leaves was not affected, potassium concentration and potassium content in different parts of rape decreased significantly under nitrogen deficiency stress.Nitrogen deficiency treatment significantly inhibited the expression of most potassium channel protein genes and three root specific potassium transporter genes in rapeseed for 14 days, indicating the effect of nitrogen deficiency on the expression of K uptake and translocation genes in Brassica napus.It may lead to the decrease of potassium content in rape plants.
【作者单位】：中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点开放实验室;
【基金】：中国农业科学院创新工程(CAAS-ASTIP-2013-OCRI) 湖北省青年基金面上项目(2015CFB478) 公益性行业(农业)科研专项(201503123-9)
【分类号】：S565.4

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