番茄泛素活化酶基因家族进化及表达模式分析

发布时间：2018-04-11 17:02

  本文选题：番茄 + 泛素活化酶基因　； 参考：《热带作物学报》2017年06期

【摘要】：泛素活化酶是蛋白泛素化所需的第一个酶,在泛素蛋白酶体途径中发挥重要作用。本文利用生物信息学方法从番茄基因组中鉴定出2个泛素活化酶(ubiquitin activating enzyme,UBA)基因,命名为Sl UBA1和Sl UBA2。序列分析表明Sl UBA1和Sl UBA2基因的CDS序列长分别为3 060和3 255 bp,分别编码1 019和1 084个氨基酸,编码蛋白分子量为114.15和120.46 ku,分别位于第6和9染色体。2个基因均含有5个内含子,编码蛋白均为酸性、疏水性蛋白;对蛋白二级结构分析发现2个UBA蛋白均以α-螺旋和无规则卷曲为主;亚细胞定位预测均定位于细胞核。序列比对和进化树分析表明番茄UBA基因与其他物种UBA基因相似性高,进化过程非常保守。番茄不同组织表达分析结果表明2个UBA基因在根系和花的表达量较高,果实成熟过程中的表达量相对较低。非生物胁迫试验结果表明:Sl UBA1基因在低温、干旱和盐胁迫下均上调表达;而Sl UBA2基因对非生物胁迫没有明显响应,这些结果表明Sl UBA1基因可能参与番茄对低温、干旱和盐胁迫的响应。
[Abstract]:Ubiquitin activating enzyme is the first enzyme protein ubiquitination required, play an important role in the ubiquitin proteasome pathway. This method from tomato genome and identified 2 ubiquitin activating enzyme (ubiquitin activating enzyme by bioinformatics, UBA) gene, named as Sl UBA1 and Sl UBA2. sequence analysis showed that the CDS sequence of Sl UBA1 Sl and UBA2 gene were performed for 3060 and 3255 BP, respectively, encoding 1019 and 1084 amino acids, encoding a protein with molecular weight of 114.15 and 120.46 Ku, respectively, located on the sixth and ninth chromosome.2 gene contains 5 introns, encoding proteins were acidic and hydrophobic protein; protein secondary structure analysis found two of 2 UBA protein with alpha helix and random coil; subcellular localization prediction were located in the nucleus. Sequence alignment and phylogenetic analysis showed that UBA gene in tomato and other species similar to UBA gene, evolution Very conservative. The expression of different tissues of tomato analysis showed that 2 UBA genes were highly expressed in roots and flowers, the expression of fruit ripening is relatively low. Abiotic stress test results show that the Sl UBA1 gene in low temperature, drought and salt stress were up-regulated; and Sl UBA2 genes to abiotic stress no obvious response, these results suggest that Sl UBA1 gene may be involved in the response of tomato to low temperature, drought and salt stress.

【作者单位】： 中国热带农业科学院南亚热带作物研究所;农业部热带果树生物学重点实验室;
【基金】：中央级公益性科研院所基本科研业务专项(No.1630062014010,1630062015017)
【分类号】：S641.2
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本文编号：1736882