油桐ALDH22A1和ALDH2C4基因克隆及其序列分析

发布时间：2018-04-14 00:27

  本文选题：油桐 + ALDH　； 参考：《经济林研究》2017年03期

【摘要】：为给油桐ALDH基因的结构与功能研究提供理论依据,以葡萄桐的近成熟种子为材料,根据油桐转录组测序结果设计引物,采用RT-PCR技术克隆了油桐ALDH22A1基因和ALDH2C4基因的全长cDNA序列。ALDH22A1的cDNA序列全长1 782 bp,编码593个氨基酸,该基因编码蛋白质的相对分子质量为65.60 kDa,理论等电点为6.62,蛋白二级结构以α螺旋为主,具有1个明显的跨膜结构,是稳定的非分泌蛋白。ALDH2C4的cDNA序列全长1 506 bp,编码501个氨基酸,该基因编码蛋白质的相对分子质量为54.82 kDa,理论等电点为6.58,蛋白二级结构以α螺旋为主,是不具有跨膜结构的膜外蛋白。BLASTp分析结果表明,这2个基因所编码序列与麻疯树、蓖麻的乙醛脱氢酶一致性最高,高达80%以上,含有醛脱氢酶基因家族的保守结构域。
[Abstract]:In order to provide theoretical basis for the study of the structure and function of ALDH gene, primer was designed based on the results of transcriptome sequencing from the near mature seeds of Paulownia vinifera.The full-length cDNA sequence of ALDH22A1 gene and ALDH2C4 gene. The cDNA sequence of ALDH22A1 was cloned by RT-PCR, encoding 593 amino acids.The protein encoded by this gene has a molecular weight of 65.60 kDa and a theoretical isoelectric point of 6.62. The secondary structure of the protein is 伪 -helix, with an obvious transmembrane structure. The cDNA sequence of the stable nonsecretory protein, ALDH2C4, is 1 506bp, encoding 501 amino acids.The relative molecular weight of the protein encoded by the gene is 54.82 kDa, the theoretical isoelectric point is 6.58, and the secondary structure of the protein is 伪 -helix. The results of the analysis of the extracellular protein. BLASTp without transmembrane structure indicate that the two genes encode the same sequence as Jatropha curcas.The highest consistency of acetaldehyde dehydrogenase in castor was over 80%, which contained the conserved domain of aldehyde dehydrogenase gene family.
【作者单位】： 中南林业科技大学经济林培育与保护省部共建教育部重点实验室;中南林业科技大学林学院;
【基金】：国家林业公益性行业科研专项重大项目(201204403)
【分类号】：S794.3
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本文编号：1746916