干旱对水稻生物钟基因和干旱胁迫响应基因每日节律性变化的影响

发布时间：2018-04-20 14:11

本文选题：干旱 + 水稻　；参考：《遗传》2017年09期

【摘要】：内源生物钟的节律运动不仅调控植物的生长发育,而且在调控植物响应和适应环境过程中发挥重要的作用。为了解水稻(Oryza sativa L.)干旱胁迫响应基因和生物钟基因在干旱条件下每日表达变化情况,本文利用实时荧光定量PCR方法研究旱稻品种IRAT109在干旱胁迫下相关基因的表达变化。结果表明,干旱胁迫导致早晨生物钟基因OsPRRs、OsLHY和OsZTL1的表达量显著下降,振幅减弱;同时导致夜晚生物钟基因OsTOC1、OsGI和OsELF3整体表达量升高,振幅增强,但对OsFKF1基因影响不大。同样,大部分水稻干旱胁迫响应基因在干旱胁迫后整体表达量显著升高,但OsDST基因表达量下降;同时大部分抗逆基因周期性表达被扰乱,但OsCIPK12、OsCDPK7和OsDREB1A依然保持24 h内震荡。本研究结果表明干旱胁迫能影响生物钟元件的基因表达,这种互相影响改变了部分基因每日的震荡变化。
[Abstract]:The rhythmic movement of endogenous biological clock not only regulates the growth and development of plants, but also plays an important role in the regulation of plant response and adaptation to the environment. To understand Oryza sativa L. The changes of daily expression of drought stress response genes and circadian clock genes were studied by real-time fluorescence quantitative PCR. The expression of IRAT109 related genes in upland rice varieties under drought stress was studied by real-time fluorescence quantitative PCR. The results showed that the expression of OsPRRsHY and OsLHY and OsZTL1 in the morning and the whole expression of OsTOC1OGI and OsELF3 in the body clock gene in the night were increased and increased under drought stress, but had little effect on the OsFKF1 gene. Similarly, the total expression of most drought stress responsive genes in rice increased significantly after drought stress, but the expression of OsDST gene decreased, while the cyclic expression of most resistant genes was disturbed, but OsCIPK12 OsCDPK7 and OsDREB1A remained oscillating within 24 hours. The results show that drought stress can affect the gene expression of clock elements, and this interaction changes the daily oscillations of some genes.
【作者单位】：上海市农业生物基因中心;
【基金】：国家科技重大专项课题项目(编号:2016ZX08001-003) 国家自然科学基金面上项目(编号:31371229) 上海市自然科学基金项目(编号:17ZR1425400)资助~~
【分类号】：Q943.2;S511

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