氟化钠对附睾形态结构及紧密连接相关基因表达的影响

发布时间：2018-04-20 16:17

本文选题：氟 + 附睾　；参考：《中国畜牧兽医》2017年04期

【摘要】：为了研究氟对小鼠附睾结构形态及紧密连接相关基因的影响,将60只健康雄性小鼠随机分成4组,对照组饮用蒸馏水,3个氟处理组分别饮用含氟化钠(NaF)25、50和100mg/L的蒸馏水。染氟60d,取小鼠股骨,采用氟离子选择电极法测其氟含量;取右侧附睾制作石蜡切片,HE染色后进行组织形态结构观察;左侧附睾提取RNA,采用实时荧光定量PCR技术(qRT-PCR)检测紧密连接相关基因的表达。结果显示,与对照组相比,氟处理组小鼠股骨氟含量随摄氟剂量的增加呈剂量依赖性,其中50、100mg/L剂量组差异极显著(P0.01);氟处理组附睾尾管腔厚度极显著增厚(P0.01);紧密连接相关基因Claudin-2 mRNA表达量极显著下降(P0.01),100mg/L剂量组ZO-1mRNA表达量显著下降(P0.05)。摄入过量NaF影响附睾形态及其紧密连接相关基因的表达,从而可能对血-附睾屏障造成损伤。
[Abstract]:In order to study the influence of fluorine on the structure of epididymis and closely linked genes in mice, 60 healthy male mice were randomly divided into 4 groups. The control group drank distilled water and 3 fluoride treatment groups drank distilled water containing sodium fluoride (NaF) 25,50 and 100mg/L respectively. Fluorine 60d was used to take the femur bone of mice and the fluoride content was measured by fluorine ion selective electrode method. The paraffin section of the epididymis was made, and the tissue morphology and structure were observed after HE staining. The left epididymis was extracted with RNA, and the expression of closely linked genes was detected by real-time fluorescence quantitative PCR (qRT-PCR). The results showed that the fluoride content of the femur in the fluorine treatment group was dose-dependent with the increase of the dose of fluorine, of which 50100mg/L was compared with the control group. The difference in the dose group was very significant (P0.01); the thickness of the caudal caudal caudal lumen in the fluorine treatment group was significantly thicker (P0.01); the expression of Claudin-2 mRNA in the closely linked gene was significantly decreased (P0.01), and the ZO-1mRNA expression in the 100mg/L dose group decreased significantly (P0.05). The blood - epididymal barrier causes damage.

【作者单位】：山西农业大学动物科技学院;
【基金】：国家自然科学基金项目(31201965) 山西农业大学人才引进创新项目(XB2010003)
【分类号】：S852.3

【相似文献】