苹果MdRGL3a基因的功能分析

发布时间：2018-04-23 14:37

  本文选题：苹果 + MdRGLa　； 参考：《园艺学报》2017年06期

【摘要】：为进一步揭示苹果(Malus×domestica)中GA信号转导机制以及MdDELLAs的生理功能,以‘嘎拉’苹果为试材,克隆基因MdRGL3a(GenBank:DQ007887),并通过在拟南芥中过量表达,分析该基因的功能。qRT-PCR结果表明,MdRGL3a在苹果不同组织中都有表达,其中在花和果实中表达量较高。在拟南芥中异位表达MdRGL3a,表现为根长和下胚轴变短,种子萌发较慢,叶片变小,植株矮化。过表达MdRGL3a拟南芥植株对赤霉素介导的生长反应敏感性降低,证明MdRGL3a是GA信号途径中的一个负调控因子。
[Abstract]:In order to further reveal the mechanism of GA signal transduction and the physiological function of MdDELLAs in Malus 脳 domestica, the gene MdRGL3a was cloned into GenBank: DQ00788787, and was overexpressed in Arabidopsis thaliana. The results of qRT-PCR showed that MdRGL3a was expressed in different tissues of apple, especially in flower and fruit. The heterotopic expression of MdRGL3a in Arabidopsis thaliana showed that the root length and Hypocotyl became shorter, the seed germination was slower, the leaves were smaller and the plants were dwarfed. Overexpression of MdRGL3a in Arabidopsis thaliana plants is less sensitive to gibberellin mediated growth response, which suggests that MdRGL3a is a negative regulator in GA signaling pathway.
【作者单位】： 山东农业大学园艺科学与工程学院作物生物学国家重点实验室国家苹果工程技术研究中心;
【基金】：农业部公益性行业科研专项(201203075-3) 教育部“创新团队发展计划”项目(IRT15R42) 山东省现代农业产业技术体系创新团队项目(SDAIT-06-03)
【分类号】：S661.1
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本文编号：1792379