烟草中过表达丹参SAMDC基因增强其抗旱性（英文）

发布时间：2018-04-23 15:03

  本文选题：过表达 + S-腺苷甲硫氨酸脱羧酶(SAMDC)　； 参考：《农业生物技术学报》2017年05期

【摘要】：S-腺苷甲硫氨酸脱羧酶(SAMDC)是多胺合成代谢中的关键酶。本研究利用RT-PCR从丹参(Salvia miltiorrhiza)中获得了全长的SAMDC序列(Gen Bank No.GU983041.1),即SmSAMDC,与其他植物物种中的SAMDC氨基酸序列具有较高的同源性。为研究多胺在植物抗旱中的作用,利用根癌农杆菌(Agrobacterium tumefaciens)介导的叶盘转化法将SmSAMDC导入到烟草(Nicotiana tabacum)中,得到稳定表达SmSAMDC的转基因的T0和T1代烟草,用以抗干旱分析。SmSAMDC在烟草中的异源表达明显地提高了转基因T0和T1代烟草的抗旱能力。生理研究发现,在干旱条件下,转SmSAMDC烟草具有更高的相对含水量(relative water content,RWC)和抗氧化酶活性;相对较低的丙二醛(malondialdehyde,MDA)和超氧阴离子自由基(O2·-)含量。并且,在干旱胁迫环境中,SmSAMDC在烟草的过表达显著地促进了转基因烟草中过氧化氢(H2O2)的积累。本研究证明过表达SmSAMDC能提高转基因烟草的抗旱能力,为SmSAMDC在其他物种的应用提供了理论依据。
[Abstract]:S-adenosine methionine decarboxylase (SAMDC) is a key enzyme in polyamine synthesis and metabolism. In this study, RT-PCR was used to obtain a full-length SAMDC sequence from Salvia miltiorrhizae, Gen. Bank No.GU983041. 1, or SmSAMDC, which has high homology with SAMDC amino acid sequences in other plant species. In order to study the effect of polyamines on drought resistance in plants, SmSAMDC was introduced into Nicotiana tabacum by Agrobacterium tumefaciens-mediated leaf disc transformation, and transgenic T _ 0 and T _ 1 tobacco with stable expression of SmSAMDC were obtained. The heterologous expression of .SmSAMDC in tobacco for drought resistance analysis significantly improved the drought resistance of transgenic T0 and T1 generation tobacco. Physiological studies showed that under drought conditions, transgenic SmSAMDC tobacco had higher relative water content (water) and antioxidant enzyme activity, lower malondialdehyde (malondialdehyde) and superoxide anion free radical (O 2 -) contents. Furthermore, the overexpression of SmSAMDC in transgenic tobacco significantly promoted the accumulation of H _ 2O _ 2 in transgenic tobacco under drought stress. This study proved that overexpression of SmSAMDC can improve the drought resistance of transgenic tobacco and provide a theoretical basis for the application of SmSAMDC in other species.
【作者单位】： 电子科技大学生命科学与技术学院/信息生物学研究中心;
【基金】：中央高校基本科研业务费专项资金(No.ZYGX2014J081) 国家自然科学基金(No.31371682)
【分类号】：Q943.2
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