XRCC1基因Arg194Trp位点多态性与胃癌铂类药物化疗敏感性的关系

发布时间：2018-04-25 14:42

本文选题：胃癌 + X射线损伤交叉互补蛋白　；参考：《山东医药》2017年08期

【摘要】：目的探讨X射线损伤交叉互补蛋白1(XRCC1)Arg194Trp位点多态性与胃癌铂类药物化疗敏感性的关系。方法选择接受铂类药物化疗的胃癌患者80例,采用聚合酶链式反应-连接酶检测反应技术检测XRCC1基因Arg194Trp位点基因型。所有患者术后接受以奥沙利铂为主的一线化疗方案,根据化疗效果分为化疗敏感者和化疗耐药者,分析XRCC1基因Arg194Trp位点多态性与化疗敏感性的关系。结果 XRCC1基因Arg194Trp位点存在Arg/Arg、Arg/Trp、Trp/Trp三种基因型,分布频率分别为42.5%(34/80)、50.0%(40/80)、7.5%(6/80);三种基因型分布满足HardyWeiberg基因遗传平衡定律,具有群体代表性。Arg/Arg、Arg/Trp、Trp/Trp基因型患者性别、年龄、组织类型、TNM分期、化疗方案等临床病理参数比较差异均无统计学意义(P均0.05)。80例患者中,化疗敏感(完全缓解+部分缓解)39例(48.8%),耐药(稳定+进展)41例(51.2%);Arg/Trp、Trp/Trp基因型者化疗敏感率均显著高于Arg/Arg者(P均0.05),Arg/Trp与Trp/Trp基因型患者化疗敏感率比较差异无统计学意义(P0.05)。结论 XRCC1基因Arg194Trp位点多态性与胃癌患者铂类药物化疗敏感性有关;检测Arg194Trp位点多态性可为胃癌化疗的个体化用药提供依据。
[Abstract]:Objective to investigate the relationship between 1(XRCC1)Arg194Trp polymorphism of X-ray injury cross-complementary protein and chemosensitivity of platinum drugs in gastric cancer. Methods A total of 80 patients with gastric cancer received chemotherapy with platinum drugs were selected to detect the genotypes of XRCC1 gene Arg194Trp loci by polymerase chain reaction-ligase assay. All patients were treated with oxaliplatin as the main first-line chemotherapy regimen. According to the effect of chemotherapy, the patients were divided into chemotherapy-sensitive and chemotherapeutic patients. The relationship between the polymorphism of Arg194Trp locus of XRCC1 gene and chemosensitivity was analyzed. Results there were three Arg / Arg / Arg / TrpTrpTrp genotypes in the Arg194Trp locus of the XRCC1 gene, and the distribution frequencies were 42.5% / 80% and 50.040 / 80%, respectively. The distribution of the three genotypes met the law of genetic balance of the HardyWeiberg gene, and the distribution of the three genotypes met the law of genetic balance of the HardyWeiberg gene. Argr / Argr Argr / ArgP / TrpTrpTrp genotype patients had sex, age, tissue type and stage of TNM. There was no significant difference in the clinicopathologic parameters of chemotherapy regimen between the two groups (P = 0.050.80). The chemosensitivity rate of 39 patients with complete remission and 41 patients with stable progression were significantly higher than that of patients with Arg/Arg. There was no significant difference in the rates of chemosensitivity between ARG / TRP and Trp/Trp genotypes (P 0.05). Conclusion the polymorphism of Arg194Trp locus of XRCC1 gene is related to the chemotherapeutic sensitivity of platinum-like drugs in gastric cancer patients, and the detection of Arg194Trp locus polymorphism may provide evidence for individualized chemotherapy of gastric cancer.
【作者单位】：海南省人民医院;福建医科大学附属第一医院;
【基金】：海南省医学科研立项课题(琼卫[2012]PT-17)
【分类号】：R735.2

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