前列腺癌ERG基因重排与蛋白表达及其与预后的关系

发布时间：2018-05-06 01:26

本文选题：前列腺癌 + ERG　；参考：《四川大学学报(医学版)》2017年01期

【摘要】：目的检测中国前列腺癌患者ETS相关基因(ERG)重排及蛋白表达情况,分析其与患者预后的关系。方法选取四川大学华西医院2009~2014年前列腺穿刺确诊的前列腺癌患者482例,采用荧光原位杂交法检测ERG基因重排情况,采用免疫组化染色法检测ERG蛋白表达情况。采用Spearman秩相关分析ERG基因重排与蛋白表达的相关性,采用χ~2检验分析ERG基因重排和蛋白表达与患者临床病理特征的关系,采用KaplanMeier法分析ERG基因重排和蛋白表达与患者预后的关系。结果 87例(18.0%)患者检出ERG基因重排,其中45例(51.7%)为转位重排,42例(48.3%)为缺失重排。74例(15.4%)患者表达ERG蛋白。ERG蛋白表达与基因重排正相关(r=0.849,P=0.000)。368例获得随访资料,ERG基因重排状态及蛋白表达水平与患者年龄、Gleason评分、初诊时前列腺特异性抗原(PSA)水平无关(P0.05),但发生远处转移及进展为去势抵抗性前列腺癌(CRPC)的病例中ERG蛋白表达率下降(P0.05)。预后分析显示ERG基因重排及蛋白表达均不能提示预后。结论 ERG基因重排状态与蛋白表达情况均不能作为前列腺癌进展为CRPC及总生存率的独立预测指标。
[Abstract]:Objective to study the relationship between ETS gene rearrangement and protein expression in Chinese patients with prostate cancer. Methods from 2009 to 2014, 482 patients with prostate cancer diagnosed by prostate puncture in Huaxi Hospital of Sichuan University were selected. The ERG gene rearrangement was detected by fluorescence in situ hybridization, and the expression of ERG protein was detected by immunohistochemical staining. Spearman rank correlation analysis was used to analyze the correlation between ERG gene rearrangement and protein expression, and 蠂 2 test was used to analyze the relationship between ERG gene rearrangement and protein expression and clinicopathological features. The relationship between ERG gene rearrangement and protein expression and prognosis was analyzed by KaplanMeier method. Results ERG gene rearrangement was detected in 87 patients. Among them, 45 cases (51.7) (42 cases with transposition rearrangement) and 74 cases with missing rearrangement (15. 4%) expressed ERG protein. ERG protein expression and gene rearrangement were positively correlated with gene rearrangement. 368 cases were followed up. The status and expression level of ERG gene rearrangement and protein expression were compared with Gleason score of patients. The expression rate of ERG protein was decreased in patients with distant metastasis and progression to castrated resistant prostate cancer (CRPC). Prognostic analysis showed that neither ERG gene rearrangement nor protein expression could predict prognosis. Conclusion neither ERG gene rearrangement nor protein expression can be used as an independent predictor of CRPC and overall survival rate in prostate cancer.
【作者单位】：四川大学华西医院病理科·病理研究室;四川大学华西医院泌尿外科;
【基金】：国家自然科学基金(No.81272820、No.81272848、No.81572540和No.81572541)资助
【分类号】：R737.25

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