甘蔗独脚金内酯生物合成关键基因ScD27的克隆与表达分析

发布时间：2018-05-07 13:31

本文选题：甘蔗 + ScD　；参考：《作物学报》2017年01期

【摘要】：独脚金内酯(strigolactones,SLs)是一类新型植物激素,D27基因是独脚金内酯生物合成途径中最上游的调控基因,且该基因调控SLs的合成是一个可逆的过程。本研究根据水稻、玉米、高粱、二穗短柄草4种禾本科作物的D27基因核苷酸序列保守区域设计引物,以甘蔗品种ROC22的c DNA为模板,利用RT-PCR和RACE技术,从甘蔗中克隆出D27基因的c DNA全长序列,命名为Sc D27,Gen Bank登录号为KP987221.1。该基因序列全长1379 bp,包含一个867 bp的完整开放阅读框(ORF),编码288个氨基酸残基。Sc D27编码的蛋白质分子量为71.58 k D,理论等电点为5.04,是一种非分泌性蛋白,主要分布于叶绿体上,该蛋白的保守区可能具有2个锌指蛋白结构域(Zn F_TAZ和Zn F_A20),且不存在信号肽;该基因编码的氨基酸序列具有较高的保守性,与高粱、谷子、大麦、短穗二柄草等禾本科植物的D27氨基酸序列相似性在70%以上;Sc D27基因在甘蔗各组织部位均有表达,其中茎尖和腋芽中表达量较高,叶、茎和根中的表达较低。此外,Sc D27基因在甘蔗茎尖中的表达受PEG、盐胁迫、磷缺乏和营养缺乏的诱导,推测Sc D27基因是甘蔗独脚金内酯生物合成途径中响应非生物胁迫的关键基因。
[Abstract]:Strigolactones sls) is a novel plant hormone, D27 gene, which is the upstream regulation gene in the biosynthesis pathway of monogolide, and it is a reversible process to regulate the synthesis of SLs. In this study, primers were designed according to the conserved region of nucleotide sequence of D27 gene of four gramineous crops, rice, maize, sorghum, and two species of grass. Using c DNA of sugarcane variety ROC22 as template, RT-PCR and RACE techniques were used. The full-length c DNA sequence of D27 gene was cloned from sugarcane and named SC D27G Bank accession number KP987221.1. The full length of the gene is 1379 BP, which contains a complete open reading frame of 867 BP, encoding 288 amino acid residues. Sc D27 encodes a protein molecular weight of 71.58 KD and a theoretical isoelectric point of 5.04. It is a non-secretory protein, mainly distributed on chloroplasts. The conserved region of this protein may have two zinc finger protein domains Zn F_TAZ and Zn FFA20, and there is no signal peptide, and the amino acid sequence of this gene is highly conserved with that of sorghum, millet and barley. The similarity of D27 amino acid sequence in Gramineae plants was more than 70%. Sc D27 gene was expressed in all tissues of sugarcane, especially in stem tip and axillary bud, but lower in leaf, stem and root. In addition, the expression of Sc D27 gene in sugarcane stem tip was induced by PEG, salt stress, phosphorus deficiency and nutrient deficiency. It is inferred that SC D27 gene is the key gene in the biosynthesis pathway of sugarcane monopodide in response to abiotic stress.
【作者单位】：云南省农业科学院甘蔗研究所/云南省甘蔗遗传改良重点实验室;
【基金】：国家自然科学基金项目(31360359) 国家现代农业产业技术体系建设专项(CARS-20-1-1) 云南省中青年学术技术带头人后备人才(2014HB038) 云南省应用基础研究计划项目(2016FB071) 重大科技专项-生物(2015ZA001) 科技创新人才计划(2014HC015)资助~~
【分类号】：S566.1

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