大豆炸荚基因GmAGL8的转基因鉴定与功能初探

发布时间：2018-05-09 01:12

本文选题：大豆 + 炸荚　；参考：《大豆科学》2017年03期

【摘要】：炸荚是大豆的一种自然特征属性,是影响大豆产量的重要因素之一。本研究以前期获得的转大豆炸荚相关基因GmAGL8的T_1代植株为材料,继续繁育获得T_2和T_3代;采用PCR和RT-q PCR检测方法对转基因植株进行基因遗传稳定性和表达情况分析;并以野生型中黄10号为对照,对大豆炸荚性状进行了鉴定分析。结果表明:转基因植株阳性率T_1代为87.5%,T_2和T_3代均达到100%,说明GmAGL8基因已基本能够在转基因后代中稳定遗传。RT-q PCR检测结果显示,转基因植株中GmAGL8基因的相对表达量都明显高于非转基因植株,且各转基因植株之间表达量具有差异性。对T_1、T_2和T_3代植株炸荚率进行了统计,不同世代转基因大豆的平均炸荚率为9.09%,而非转基因大豆炸荚率为83.3%,转基因与非转基因大豆之间炸荚率存在显著差异。综上所述,GmAGL8基因已基本实现在大豆转基因后代中稳定遗传并正常表达,表型鉴定结果初步证明了GmAGL8基因与大豆炸荚性状相关。
[Abstract]:Pod bombing is a natural characteristic of soybean and one of the important factors affecting soybean yield. In this study, the first generation of transgenic soybean pod-related gene GmAGL8 was used as the material to continue to breed and obtain T2 and T _ (3) generations, and the genetic stability and expression of the transgenic plants were analyzed by PCR and RT-q PCR detection methods. The wild type Zhonghuang 10 was used as control to identify and analyze the characters of soybean pod. The results showed that the positive rate of transgenic plants was 87.5% in the first generation and 100 in the third generation of T _ S _ 2 and T _ T _ 2, indicating that the GmAGL8 gene had been able to be stably inherited in the transgenic progenies by .RT-q PCR. The relative expression of GmAGL8 gene in transgenic plants was significantly higher than that in non-transgenic plants, and there were differences among the transgenic plants. According to the statistics of pod-fried rate of T _ 1 / T _ 2 and T _ S _ 3, the average pod rate of transgenic soybean was 9.09, while that of non-transgenic soybean was 83.3%. There was significant difference between transgenic soybean and non-transgenic soybean. In conclusion, GmAGL8 gene has been stably inherited and normally expressed in soybean transgenic progenies. Phenotypic identification results showed that the GmAGL8 gene was related to soybean pod traits.
【作者单位】：北京农学院植物科学技术学院;北京农学院生物科学与工程学院;中华人民共和国农业部华北都市农业重点实验室;
【基金】：国家高技术研究发展计划“863计划”(2012AA101106)
【分类号】：S565.1

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