荔枝ACS1基因的分离及其与幼果脱落的关系

发布时间：2018-05-18 03:05

本文选题：荔枝 + ACC合成酶　；参考：《果树学报》2017年07期

【摘要】：【目的】植物中ACC合成酶(ACC synthase,ACS)和ACC氧化酶(ACC oxidase,ACO)是调控乙烯形成的关键酶。荔枝ACO基因已分离获得,在此基础上,笔者进一步从荔枝中分离出ACS基因,进而分析该基因与荔枝幼果脱落的关系。【方法】通过RT-PCR和RACE扩增技术相结合的方法分离荔枝ACS基因;利用遮阴、环剥摘叶和100 mg·L-1NAA处理促进荔枝幼果脱落,进而用实时荧光定量PCR分析荔枝ACS基因与幼果脱落的关系。【结果】首次从荔枝中分离出ACS基因,命名为Lc-ACS1。Lc-ACS1推导的氨基酸序列与多种植物的ACS蛋白有较高的同源性,其中与甜橙和蓖麻的同源性最高,为76%。该基因包含ACS基因特有的11个不变氨基酸残基和7个保守区。遮阴、环剥摘叶和100 mg·L-1NAA处理都可以显著促进荔枝幼果脱落,且环剥摘叶后幼果乙烯释放量高峰出现在第2天。Lc-ACS1基因在任何一种促进荔枝幼果脱落过程中的表达量都高于对照,且基因表达量的高峰都早于相对落果率高峰。【结论】LcACS1基因可能是调控荔枝幼果脱落中的关键基因。
[Abstract]:[objective] ACC synthase (ACC synthase) and ACC oxidase (ACC oxidase) are the key enzymes to regulate ethylene formation in plants. ACO gene was isolated from litchi, and ACS gene was further isolated from litchi. The relationship between the gene and litchi young fruit abscission was analyzed. [methods] Litchi ACS gene was isolated by RT-PCR and RACE amplification, and the shedding of litchi young fruit was promoted by shading, girdling and 100mg / L-1NAA treatment. The relationship between ACS gene and young fruit abscission was analyzed by real-time fluorescence quantitative PCR. [results] the ACS gene was isolated from litchi for the first time, and the amino acid sequence derived by Lc-ACS1.Lc-ACS1 had high homology with ACS protein of many plants. The highest homology with sweet orange and castor is 76%. This gene contains 11 invariant amino acid residues and 7 conserved regions of ACS gene. Shading, girdling and 100mg L-1NAA treatment could significantly promote the shedding of litchi young fruit, and the peak of ethylene release of young fruit appeared on the 2nd day, the expression of Lc-ACS1 gene in any kind of litchi young fruit shedding was higher than that of control. The peak of gene expression was earlier than the peak of relative fruit drop rate. [conclusion] LcACS1 gene may be the key gene to regulate the shedding of litchi young fruit.
【作者单位】：岭南师范学院基础教育学院;华南农业大学园艺学院·广东省荔枝工程技术研究中心;
【基金】：农业部农业科研杰出人才及“荔枝花果发育理论与栽培技术创新团队”项目国家现代农业产业技术体系(CARS-33-11)
【分类号】：S667.1

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