玉米ZmBRI1基因的克

发布时间：2018-05-25 06:57

  本文选题：玉米 + ZmBRI　； 参考：《作物学报》2017年09期

【摘要】：油菜素内酯(BRs)是一种重要的甾族类激素,在植物的生长过程中起着重要的作用。本研究利用同源克隆的方法,从玉米B73自交系中获得了一个新的油菜素内酯受体蛋白编码基因ZmBRI1,该基因全长为3369 bp,编码1122个氨基酸。亚细胞定位分析表明,ZmBRI1蛋白定位于细胞膜上,而且ZmBRI1在各个玉米的各个组织器官中都有表达,其中在幼嫩的组织中表达最高。利用转基因技术将ZmBRI1导入BR不敏感突变体bri1-5中,获得的转基因植株修复了其表型,特别是植株高度、叶片形态和果荚大小。与突变体bri1-5比较,油菜素内酯(brassinolide,BL)处理显著抑制转基因株系根系生长;丙环唑(propiconazole,Pcz)处理显著抑制了下胚轴生长;同时转基因株系DWF4和CPD基因的表达量下降。此外,在野生型(Ws)中过表达ZmBRI1,显著提高了拟南芥在ABA抑制条件下的种子萌发率和植株生长,下调了ABA响应基因RD29A、RD29B、ABI5和RAB18的表达。因此,ZmBRI1不仅参与了植物的形态建成和BR的信号传导,而且参与调控了植物对ABA信号的响应。
[Abstract]:Brassinolide (BRs) is an important steroid hormone, which plays an important role in plant growth. In this study, a novel rapesinolactone receptor protein encoding gene ZmBRI1 was obtained from maize B73 inbred line by homologous cloning. The gene is 3369 BP and encodes 1122 amino acids. Subcellular localization analysis showed that ZmBRI1 protein was located on the cell membrane, and ZmBRI1 was expressed in all tissues and organs of maize, and the highest expression was found in young tissues. ZmBRI1 was introduced into Br insensitive mutant bri1-5 by transgenic technique. The transgenic plants repaired their phenotypes, especially plant height, leaf morphology and pod size. Compared with the mutant bri1-5, Brassinolide (BLL) treatment significantly inhibited the root growth of transgenic lines, the propiconazoleoledol (Pcz) treatment significantly inhibited the Hypocotyl growth, and the expression of DWF4 and CPD genes decreased in the transgenic lines. In addition, the overexpression of ZmBRI1 in wild type Ws1 significantly increased the seed germination rate and plant growth of Arabidopsis thaliana under the condition of ABA inhibition, and down-regulated the expression of ABA response gene RD29A, RD29B, ABI5 and RAB18 in Arabidopsis thaliana. ZmBRI1 is involved not only in plant morphogenesis and Br signal transduction, but also in plant response to ABA signal.
【作者单位】： 植物生长调节剂教育部工程研究中心/中国农业大学农学与生物技术学院农学系;
【基金】：引进国际先进农业科学技术计划(948计划)项目(2011-G19)资助~~
【分类号】：S513
，

本文编号：1932517