金鱼草丙二烯环化酶基因AmAOC的克隆及表达分析

发布时间：2018-05-27 17:10

本文选题：金鱼草 + AOC基因　；参考：《分子植物育种》2017年09期

【摘要】：作为植物信号分子的茉莉酸(JA)参与植物的发育与代谢过程,丙二烯环化酶(AOC)是JA合成途径的关键酶。在本研究中,利用同源克隆结合RACE技术从金鱼草中克隆AOC基因,命名为Am AOC。Am AOC基因开放阅读框长762 bp,由254个氨基酸残基组成,该基因包含一个Alleneoxcyc结构域,有两个内含子和三个外显子。同源比对分析结果表明Am AOC与其他植物的AOC有较高的同源性。Am AOC基因在不同组织中的表达分析发现金鱼草花中的Am AOC相对表达量较高,不同花期金鱼草RT-PCR表明,花蕾期的Am AOC相对表达量最高,盛花期的不同花器官荧光定量PCR分析表明,金鱼草花筒和花萼中Am AOC相对表达量较高。
[Abstract]:JAA, a plant signaling molecule, is involved in the development and metabolism of plants. Allyl cyclase (AOC) is the key enzyme in JA synthesis pathway. In this study, the AOC gene was cloned by homologous cloning and RACE from Goldfish. The gene was named am AOC.Am AOC open reading frame 762 BP and consisted of 254 amino acid residues. The gene contains a Alleneoxcyc domain. There are two introns and three exons. The results of homology analysis showed that am AOC had high homology with other plants' AOC. The expression of am AOC gene in different tissues was higher than that in other plants. The relative expression of am AOC in flower bud stage was the highest, and the fluorescence quantitative PCR analysis of different floral organs at full flowering stage showed that the relative expression of am AOC was higher in the tube and calyx of Astragalus mandshurica.
【作者单位】：北京农学院园林学院;北京林果业生态环境功能提升协同创新中心;
【基金】：国家自然科学基金(31640070) 北京市自然科学基金(8132005) 北京市属高等学校创新团队建设与教师职业发展计划项目(IDHT20150503) CEFF-PXM(2016-014207-000038)共同资助
【分类号】：Q943.2;S682.19

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