雌蕊缺失茶树花3个发育期的数字基因表达谱分析

发布时间：2018-05-27 17:35

本文选题：茶树花 + 雌蕊缺失　；参考：《茶叶科学》2017年01期

【摘要】：以雌蕊缺失茶树花为试材,利用转录组、数字基因表达谱技术,研究了雌蕊缺失茶树花的花芽、花蕾、花3个时期相关基因的表达规律。结果发现,雌蕊缺失茶树花生长发育过程中进行着各种旺盛的生物合成和代谢活动。生长素信号转导途径的6个基因和ABCDE类识别基因的A类、C类和E类可能与茶树花的雌蕊缺失和雄蕊发育密切相关,并且基因调控过程较复杂;WUS基因中WUS2和WUS8下调可能调控了C类和E类基因,从而导致雌蕊的缺失;KNOX家族中,未检测到KNOXⅡ的同源基因,KNOX I类同源基因下调表达和缺失可能减弱了茶树花雌蕊心皮的启动和雌蕊边缘组织的生长。可以看出,本研究通过数字基因表达谱分析,初步了解了雌蕊缺失茶树花发育前后的网络途径,为后期对茶树花雌蕊缺失和雄蕊发育的研究,探明茶树花不育和性别决定基因的分子机制提供理论依据。
[Abstract]:Taking the pistil missing tea tree as a test material, using the transcriptional group and digital gene expression spectrum technique, the expressions of the related genes in the flower buds, buds and flowers of the pistils were studied in 3 periods. The results showed that the growth process of the pistil missing tea tree was carried out with various vigorous biosynthesis and metabolic activities. The 6 genes of the diameter and the class a of the ABCDE class identification genes, C and E may be closely related to the pistil deletion and stamen development of the tea flower, and the gene regulation process is more complex. The WUS gene may regulate the C class and E gene in the WUS gene and lead to the absence of the pistil, and the KNOX family has not detected the homologous gene of KNOX II, KNOX I. The down-regulated expression and deletion of the homologous genes may weaken the initiation of pistil carpel and the growth of the pistil marginal tissue of the Camellia sinensis. It can be seen that the network approach before and after the development of the pistil missing tea tree flower was preliminarily understood by the digital gene expression spectrum analysis. The molecular basis of sterility and sex determining genes in tree flower is provided.
【作者单位】：云南省农业科学院茶叶研究所云南省茶树种质资源创新与配套栽培技术工程研究中心;安徽农业大学茶树生物学与资源利用国家重点实验室;
【基金】：国家自然科学基金(31560220;31460216) 云南省人才培养计划(2015HB105) 茶树生物学与资源利用国家重点实验室开放基金(SKLTOF20150105)
【分类号】：S571.1

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