苹果MdMYB2基因的克隆及功能鉴定

发布时间：2018-05-27 17:37

本文选题：苹果 + MdMYB　；参考：《园艺学报》2017年12期

【摘要】：以‘嘎拉’苹果为材料,采用同源克隆和PCR技术分离了苹果基因MdMYB2(基因序列号:MDP0000823458)。MdMYB2的开放阅读框(ORF)长度为873 bp,编码含有290个氨基酸的蛋白,预测其蛋白质分子量为32.92 kD,等电点为4.91。系统进化树分析显示,苹果MYB2与梨MYBL2亲缘关系最近,同源性最高。组织表达分析表明,MdMYB2在苹果的各个组织均有表达,在茎和果实中表达相对较高。MdMYB2的表达明显受盐胁迫的诱导。构建了MdMYB2的表达载体,并通过农杆菌介导的遗传转化得到了转基因苹果愈伤和转基因拟南芥植株。抗性试验表明,过量表达MdMYB2明显提高了转基因苹果愈伤对盐胁迫的抗性。此外,异位表达MdMYB2也能提高拟南芥对盐胁迫的抗性,以上试验结果表明MdMYB2在植物盐胁迫响应中发挥重要作用。
[Abstract]:Using 'Gala' apple as material, MdMYB2 gene was isolated by homologous cloning and PCR technique. The ORFlength of MdMYB2 (gene sequence number: MDP00823458N. MdMYB2) was 873 BP, encoding 290-amino acid protein. The molecular weight of MdMYB2 gene was 32.92 kDand the isoelectric point was 4.91. Phylogenetic tree analysis showed that apple MYB2 had the highest homology with pear MYBL2. Tissue expression analysis showed that MdMYB2 was expressed in all tissues of apple, and the expression of MdMYB2 in stem and fruit was relatively high. The expression of MdMYB2 was obviously induced by salt stress. The expression vector of MdMYB2 was constructed and transgenic apple callus and transgenic Arabidopsis plants were obtained by Agrobacterium tumefaciens mediated genetic transformation. The results of resistance test showed that overexpression of MdMYB2 significantly increased the resistance of transgenic apple callus to salt stress. In addition, the heterotopic expression of MdMYB2 could also increase the resistance of Arabidopsis thaliana to salt stress. The results indicated that MdMYB2 played an important role in the response of plants to salt stress.
【作者单位】：山东农业大学园艺科学与工程学院作物生物学国家重点试验室;
【基金】：国家自然科学基金项目(31601742,31772275,31471854) 山东省产业体系岗位专家项目(SDAIT-06-03)
【分类号】：S661.1

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