DPF2基因RNA干扰对人胰腺癌细胞PANC-1增殖、凋亡和细胞周期的作用研究

发布时间：2018-06-02 06:55

本文选题：DPF + RNA干扰　；参考：《中国药理学通报》2017年05期

【摘要】：目的已知DPF2参与白血病以及肿瘤的发生,但是DPF2是否参与胰腺癌发生和进展还不清楚,因此观察了DPF2基因RNA干扰对胰腺癌细胞系PANC-1细胞增殖、凋亡和细胞周期的影响。方法采用慢病毒介导的DPF2基因RNA干扰敲低PANC-1细胞的DPF2表达,通过克隆形成实验和MTT实验检测DPF2基因RNA干扰对PANC-1细胞增殖的作用,通过流式细胞术检测DPF2基因RNA干扰对PANC-1细胞凋亡和细胞周期的作用。结果慢病毒介导的DPF2基因RNA干扰中剂量和高剂量(2μL和4μL)使PANC-1细胞的DPF2表达明显降低。与阴性对照组比较,DPF2基因RNA干扰明显抑制PANC-1细胞活力和克隆形成,还促进PANC-1的凋亡。此外,DPF2基因RNA干扰引起细胞周期的S期阻滞,明显减少G2/M周期的细胞数量。结论 DPF2可能参与胰腺癌细胞PANC-1的增殖、凋亡过程和细胞周期的调控,通过慢病毒介导的DPF2基因RNA干扰敲低DPF2蛋白表达,可能为寻找潜在的抗胰腺癌的新方法提供实验依据。
[Abstract]:Objective it is known that DPF2 is involved in the occurrence of leukemia and tumor, but it is not clear whether DPF2 participates in the development and progression of pancreatic cancer. Therefore, the effects of DPF2 gene RNA interference on the proliferation, apoptosis and cell cycle of pancreatic cancer cell line PANC-1 cells are observed. Methods the DPF2 expression of the DPF2 gene RNA interfered by the lentivirus mediated DPF2 gene is disturbed. The effect of DPF2 gene RNA interference on the proliferation of PANC-1 cells was detected by cloning and MTT experiments. The effect of DPF2 gene RNA interference on the apoptosis and cell cycle of PANC-1 cells was detected by flow cytometry. The results showed that the dose and high dose of the lentivirus mediated RNA interference in DPF2 gene (2 mu L and 4 mu L) reduced the expression of the PANC-1 cells obviously. Lower. Compared with the negative control group, the DPF2 gene RNA interference significantly inhibited the activity of PANC-1 cells and the formation of clones, and also promoted the apoptosis of PANC-1. In addition, the DPF2 gene RNA interference caused the S phase block of the cell cycle and significantly reduced the number of cells in the G2/M cycle. Conclusion DPF2 may be involved in the proliferation, apoptosis and cell cycle of the PANC-1 of the pancreatic cancer cells. Regulation of DPF2 protein expression by lentivirus mediated DPF2 gene RNA interference may provide experimental evidence for finding new potential anti pancreatic cancer methods.
【作者单位】：安徽医科大学基础医学院;安徽医科大学临床药理研究所;美国马里兰大学生物医学工程技术中心;
【基金】：国家自然科学基金资助项目(No 31271159) 安徽省高校学科拔尖人才学术资助重点项目(No gxbj ZD2016030)
【分类号】：R735.9

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