玉米热激转录因子基因ZmHsf25的克

发布时间：2018-06-02 07:32

  本文选题：玉米 + 热激转录因子基因ZmHsf　； 参考：《作物学报》2017年07期

【摘要】：植物热激转录因子(heat shock transcription factor,Hsf)对下游热激蛋白基因及耐热性相关基因的表达起关键调控作用。玉米中至少有30个Hsf家族成员,其中B族有7个。在前期对玉米A1亚族热激转录因子基因ZmHsf06克隆及特性、抗旱耐热性功能分析的基础上,从玉米幼叶中克隆到B族热激转录因子基因ZmHsf25的完整编码序列,序列全长957 bp,编码318个氨基酸残基。蛋白质序列含有DNA结合结构域、核定位信号序列和核输出信号序列,同源分析结果显示,ZmHsf25与高粱Sb06g025710的相似性最高,达92%。荧光定量分析表明,正常生长条件下,ZmHsf25在玉米多个组织器官中表达,幼嫩花粉中高表达,幼嫩根系和雌穗中表达量较低。42℃热胁迫能显著上调根系和叶片ZmHsf25的表达,叶片中上升幅度高于根系,处理50 min达到峰值;正常条件下,水杨酸和H2O2处理均使根系和叶片ZmHsf25表达下调,而热激条件下却表现为显著上调。通过在洋葱表皮细胞中瞬时表达并观察GFP荧光,确定ZmHsf25定位在细胞核。通过转化酵母并进行热胁迫处理发现,热胁迫同时降低正常和转ZmHsf25酵母的耐热性,但与转空载体对照相比,转基因酵母的耐热性更强。推测ZmHsf25参与玉米热胁迫响应和花粉的发育过程,可能是水杨酸热激信号转导途径的下游组分。研究结果为进一步探明玉米热激转录因子家族成员的特性与功能提供了依据。
[Abstract]:Heat shock transcription factor-Hsf) plays a key role in regulating the expression of heat shock protein genes and heat tolerance related genes. There are at least 30 Hsf family members in maize, including 7 in B group. Based on the cloning and characterization of heat shock transcription factor gene (ZmHsf06) of subfamily A1 and the function analysis of drought resistance and heat tolerance, the complete coding sequence of group B heat shock transcription factor gene ZmHsf25 was cloned from young leaves of maize. The sequence was 957 BP, encoding 318 amino acid residues. The protein sequence contained DNA binding domain, nuclear localization signal sequence and nuclear output signal sequence. The homology analysis showed that ZmHsf25 had the highest similarity with sorghum Sb06g025710, and reached 92 points. Fluorescence quantitative analysis showed that ZmHsf25 was expressed in several tissues and organs of maize under normal growth conditions, high in young pollen, and significantly up-regulated ZmHsf25 expression in roots and leaves under low heat stress at 0.42 鈩，

本文编号：1967943