IL-18基因联合二萜生物碱对舌鳞癌细胞的增殖抑制与促凋亡作用

发布时间：2018-06-03 01:45

本文选题：白细胞介素(IL-) + 二萜生物碱　；参考：《西安交通大学学报(医学版)》2017年02期

【摘要】：目的探讨人IL-18基因联合二萜生物碱对舌鳞癌细胞Tscca的增殖抑制与促凋亡作用。方法构建pEGFPN3-IL-18质粒,转染舌鳞癌细胞Tscca。荧光显微镜下观察质粒的转染效率;MTT检测pEGFPN3-IL-18联合二萜生物碱对舌鳞癌细胞Tscca生长的影响;流式细胞术检测细胞凋亡情况;蛋白质免疫印迹法检测细胞信号调节激酶Akt/p-Akt的蛋白水平。结果转染pEGFPN3-IL-18的舌鳞癌细胞Tscca较未转染细胞凋亡数增加,差异有统计学意义(P0.05);0.2、0.4、0.6mg/mL二萜生物碱培养舌鳞癌细胞,随浓度增加,细胞凋亡数增多,差异有统计学意义(P0.05);IL-18与二萜生物碱联合对Tscca细胞作用48h后,较单独使用二萜生物碱的增殖抑制作用明显,且呈浓度依赖性,差异有统计学意义(P0.05);IL-18联合二萜生物碱还可剂量依赖性地降低p-Akt的蛋白水平。结论pEGFPN3-IL-18联合二萜生物碱对舌鳞癌细胞Tscca有协同抑制作用。
[Abstract]:Objective to investigate the effects of human IL-18 gene combined with diterpene alkaloid on the proliferation and apoptosis of tongue squamous carcinoma cell line Tscca. Methods pEGFPN3-IL-18 plasmid was constructed and transfected into Tscca cells. The effect of pEGFPN3-IL-18 combined with diterpene alkaloid on the growth of Tscca was observed under fluorescence microscope, and apoptosis was detected by flow cytometry. The protein level of signal-regulated kinase (Akt/p-Akt) was detected by Western blot. Results the number of apoptosis of Tscca transfected with pEGFPN3-IL-18 was higher than that of untransfected cells. The difference was statistically significant (P 0.05). The apoptosis of tongue squamous cell carcinoma cells was increased with the increase of the concentration of diterpenoid alkaloid (0.6 mg / mL). There was a significant difference in the inhibition of proliferation of Tscca cells by P0.05- IL-18 and diterpene alkaloids in a dose-dependent manner compared with those treated with diterpene alkaloids alone for 48 h. The difference was statistically significant (P 0.05) combined with diterpenoid alkaloids could also decrease the protein level of p-Akt in a dose-dependent manner. Conclusion pEGFPN3-IL-18 combined with diterpene alkaloid has synergistic inhibitory effect on Tscca of tongue squamous carcinoma cells.
【作者单位】：锦州医科大学研究生院;锦州医科大学附属第一医院变态反应与临床免疫研究中心;锦州医科大学附属第二医院口腔修复科;
【基金】：辽宁医学院校长基金-奥鸿博泽研究生科技创新基金(No.AH2015007)~~
【分类号】：R739.86

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