拜城油鸡肌肉生长抑制素基因多态性与生产性能的相关性研究

发布时间：2018-06-09 04:18

本文选题：拜城油鸡 + MSTN基因　；参考：《中国畜牧兽医》2017年04期

【摘要】：试验旨在研究肌肉生长抑制素(myostatin,MSTN)基因在拜城油鸡中的多态性及其与生产性能的关系,探寻可用于拜城油鸡选育的分子遗传标记。选取96只6周龄的拜城油鸡公鸡,随机分为3组,每组32只,分别在12、18和24周龄时对其生产性能指标进行测定,同时采用PCR-RFLP方法检测MSTN基因的多态性,分析MSTN基因多态性与拜城油鸡生产性能的关系。结果显示,在拜城油鸡公鸡中MSTN基因存在多态性,检测到3种基因型:AA、GA、GG,GA为优势基因型,等位基因A为优势等位基因,不符合哈代-温伯格平衡定律(P0.05)。在12周龄时,AA基因型个体的体重、屠体重、半净膛重、全净膛重、胸肌重和腿肌重均显著高于GA基因型个体(P0.05);在18周龄时,GA基因型个体的体重、胸肌重和腿肌重均显著高于GG基因型个体(P0.05);在24周龄时,AA基因型个体的体重、屠体重和半净膛重均极显著高于GG基因型个体(P0.01),全净膛重和腿肌重显著高于GG基因型个体(P0.05),GA基因型个体的体重、屠体重、半净膛重和腿肌重均显著高于GG基因型个体(P0.05),其余指标在不同周龄不同基因型个体间差异不显著(P0.05)。MSTN基因多态性与拜城油鸡部分生产性能指标密切相关,推测该基因可以作为拜城油鸡选育的分子遗传标记之一。
[Abstract]:The aim of the experiment was to study the polymorphism of myostatinine (MSTN) gene and its relationship with production performance in Baicheng oil chicken, and to explore the molecular genetic markers that can be used in breeding of Baicheng oil chicken. 96 6-week-old Beicheng fowls were randomly divided into 3 groups, 32 in each group, and their production performance indexes were measured at the age of 12 ~ 18 and 24 weeks respectively. The polymorphism of MSTN gene was detected by PCR-RFLP. To analyze the relationship between the polymorphism of MSTN gene and the performance of Baicheng fowl. The results showed that there was polymorphism of MSTN gene in Baicheng chicken cock. Three genotypes were detected as dominant genotype and allele A as dominant allele, which did not accord with Hardy-Weinberg equilibrium law (P0.05). At the age of 12 weeks, the body weight of AA genotype individuals, slaughtering weight, half bore weight, full weight, breast muscle weight and leg muscle weight were significantly higher than those of GA genotype individuals, and at 18 weeks old, the weight of GA genotype individuals was significantly higher than that of GA genotype individuals, and the weight of GA genotype individuals at 18 weeks of age was significantly higher than that of GA genotype individuals. The weight of pectoral muscle and leg muscle were significantly higher than those of GG genotype, and the weight of AA genotype was significantly higher than that of GG genotype at 24 weeks of age. The carcass weight and half bore weight of GG genotype individuals were significantly higher than those of GG genotype individuals (P 0.01), and the whole bore weight and leg muscle weight were significantly higher than those of GG genotype individuals with GG genotype P0.05 and GA genotype, and the slaughtering weight was significantly higher than that of GG genotype individuals. The weight of half bore and leg muscle were significantly higher than those of GG genotype individuals (P 0.05). There was no significant difference in other indexes among individuals of different genotypes at different weeks of age. The polymorphism of P0.05U. MSTN gene was closely related to some production performance indexes of Bicheng oil chickens. It is speculated that this gene can be used as one of the molecular genetic markers in the breeding of Baicheng oil chicken.
【作者单位】：新疆农业大学动物科学学院;
【基金】：国家自然科学基金(31260550)
【分类号】：S831

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