IGF2、H-FABP和RPL9基因在豫南黑猪和三元杂交猪中的差异表达

发布时间：2018-06-09 04:33

本文选题：豫南黑猪 + 三元杂交猪　；参考：《中国畜牧杂志》2017年07期

【摘要】：本研究旨在探析IGF2、H-FABP和RPL9基因在猪不同组织和两种猪间的表达差异。选用体重70 kg左右的杜×长×大三元杂交猪和豫南黑猪,分别采集其心、肝、脾、肺、肾、腹部脂肪和背部皮下脂肪,进行总RNA提取及不同基因的荧光定量PCR实验。结果表明:在豫南黑猪心脏、肝脏和脾脏组织中IGF2基因的转录水平约为三元杂交猪的225.0倍(P0.05);豫南黑猪心脏、脾脏和皮下组织中H-FABP基因的转录水平高于三元杂交猪,其中脾脏组织H-FABP的表达量约为三元杂交猪的6.8倍(P0.05),而三元杂交猪肺脏和腹部脂肪H-FABP表达量约为豫南黑猪的2.7倍(P0.05);RPL9基因在豫南黑猪心、肝、肺和肾组织中表达量高于三元杂交猪,在脾脏、皮下脂肪和腹部脂肪中表达量低于三元杂交猪内表达量,其中皮脂中的表达量差异最大,三元杂交猪约为豫南黑猪的4.9倍(P0.05)。综上可知,两种猪不同组织间IGF2、H-FABP和RPL9基因的表达差异显著。
[Abstract]:The purpose of this study was to investigate the difference of IGF2H-FABP and RPL9 gene expression in different tissues and between two kinds of pigs. The heart, liver, spleen, lung, kidney, abdominal fat and subcutaneous fat were collected from 70 kg DU 脳 Chang 脳 Da Ternary Hybrid Pig and Southern Henan Black Pig respectively. The total RNA was extracted and the fluorescence quantitative PCR was performed for different genes. The results showed that the transcription level of IGF2 gene in heart, liver and spleen of southern Henan black pig was about 225.0 times of that of three-way crossbred pig, and the transcription level of H-FABP gene in heart, spleen and subcutaneous tissue of south Henan black pig was higher than that in three-way crossbred pig. The expression of H-FABP in spleen was 6.8 times higher than that in three-way crossbred pigs, while the expression of H-FABP in lung and abdominal fat was 2.7 times of that in black pigs in southern Henan. The expression of RPL9 gene in heart, liver, lung and kidney of black pigs in southern Henan was higher than that in three-way crossbred pigs. The expression of sebum in spleen, subcutaneous fat and abdominal fat was lower than that in three-way crossbred pigs, and the difference in sebum expression was greatest, and the expression of sebum in three-way crossbred pigs was about 4.9 times as high as that in southern Henan black pigs (P 0.05). In conclusion, there were significant differences in the expression of IGF2H-FABP and RPL9 between the two kinds of pig tissues.
【作者单位】：河南农业大学牧医工程学院;
【分类号】：S828.2

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