MITFa及TYR基因在红色锦鲤体色发生不同阶段的表达分析

发布时间：2018-06-22 19:42

本文选题：红色锦鲤 + MITFa　；参考：《水产科学》2017年02期

【摘要】：本研究描述了红色锦鲤从出膜到体色形成的过程,总结和归纳不同发育阶段体色变化异同点,筛选出6个体色变化较显著时期,分别为1、2、3、4、12、48日龄。利用荧光定量PCR分析MITFa及TYR基因在红色锦鲤6个体色变化时期的表达情况。结果显示,MITFa基因在1日龄时表达量最高,显著高于48日龄时(P0.05),极显著高于其他4个时期(P0.01)。48日龄时表达量次之,亦极显著高于2、3、4、12日龄4个时期(P0.01)。2、3、4、12日龄4个时期MITFa基因表达量较低且不存在显著差异(P0.05)。TYR基因在1日龄时表达量最高(P0.01),4日龄时表达量显著高于12、48日龄(P0.05),与2、3日龄不存在显著差异(P0.05)。TYR基因表达量在2、3、12、48日龄4个时期差异不显著(P0.05)。MITFa和TYR基因在红色锦鲤体色形成过程中,表达水平整体呈现降低的趋势,其中MITFa基因表达量表现为先降后升,TYR基因则先降后升再降。以上结果显示MITFa、TYR基因与锦鲤体色形成具有一定相关性,但MITFa和TYR基因在体色发生中的相互作用还有待进一步研究证实。
[Abstract]:In this study, the process of color formation from membrane to body color of red koi was described, and the similarities and differences of body color changes in different developmental stages were summarized and summarized. The expression of MITFa and Tyr genes in red koi 6 individuals was analyzed by fluorescence quantitative PCR. The results showed that the expression of MITFa gene was the highest at the age of 1 day, significantly higher than that at the age of 48 days (P0.05), and significantly higher than that of the other four periods (P0.01). The expression level of MITFa gene was significantly higher than that in 4 days (P0.01). 2TYR 4 days old (P0.01), and the expression of MITFa gene was not significantly different (P0.05). TYR gene expression was significantly higher than that of 1248 days (P0.05) at the first day of age (P0.01) and no significant difference was found between the 4 days of age and the 23 days of age (P0.05), the expression of TYR gene was significantly higher than that of 1248 days of age (P0.05), and the expression of TYR gene was significantly higher than that of 1248 days of age (P0.05). There was no significant difference in the expression of TYR gene in 4 periods of age (P0.05). MITFa and TYR genes in red koi carp (P0.05). The expression level of MITFa gene decreased firstly and then increased and then decreased. These results suggest that MITFaTYR gene is related to body color formation of koi, but the interaction of MITFa and TYR genes in the development of body color still needs to be further studied.
【作者单位】：河南师范大学水产学院;
【基金】：国家自然科学基金资助项目(31402294) 国家级大学生创新创业训练计划项目(201410476006) 河南省国际科技合作计划项目(152102410040) 河南师范大学博士科研启动费支持项目(qd13056)
【分类号】：S917.4

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