粗山羊草CCT家族基因序列分析及激素响应

发布时间：2018-10-18 09:09

【摘要】：开花是植物生长发育的重要过程。CCT家族基因在植物中广泛存在,参与植物花期的调控过程。该文从粗山羊草(Aegilops tauschii)全基因组中分离出26个CCT基因,它们分布于7对染色体上,按照排列顺序将其命名为AetCCT1 26。AetCCT蛋白分子量介于14.9 kDa(AetCCT3) 83.2 kDa(AetCCT12)之间,其中有25个蛋白包含完整的CCT保守结构域。系统发育分析显示,12对粗山羊草/乌拉尔图小麦(Triticum urartu)CCT蛋白和9对粗山羊草/水稻(Oryza sativa)CCT蛋白为直系同源蛋白。通过公共数据的数字表达分析表明,AetCCT具有组织特异性和组成型2种表达形式,其中AetCCT3、AetCCT4、AetCCT7及AetCCT9等9个基因在大部分组织中都有表达,而AetCCT15、AetCCT21和AetCCT25等基因分别在种子、叶和根等少数组织中特异表达。AetCCT家族可以响应不同外源激素,施用激素24小时和72小时后各成员对激素响应整体表现一致,但不同成员对于不同激素的响应存在差异,表明该家族成员在功能和行使方式等方面具有一定的多样性,可能参与不同生长发育过程。光照条件影响AetCCT的表达,说明光照和春化作用是影响与调控该家族基因表达的重要因素。研究结果有助于探索小麦(T.aestivum)进化、驯化和演变的规律,以及认识重要农艺性状的形成与互作网络。
[Abstract]:Flowering is an important process of plant growth and development. CCT family genes exist widely in plants and participate in the regulation of plant flowering. In this paper, 26 CCT genes were isolated from the whole (Aegilops tauschii) genome of Leymus chinensis. They were distributed on 7 pairs of chromosomes. They were named AetCCT1 26.AetCCT protein with molecular weight between 14.9 kDa (AetCCT3) and 83.2 kDa (AetCCT12). Among them, 25 proteins contain complete conserved domain of CCT. Phylogenetic analysis showed that 12 pairs of (Triticum urartu) CCT protein and 9 pairs of (Oryza sativa) CCT protein of Leymus chinensis / Ulaertu were direct homologous proteins. The results of digital expression analysis of common data showed that AetCCT had two expression forms: tissue specificity and constitutive type. Among them, 9 genes, such as AetCCT3,AetCCT4,AetCCT7 and AetCCT9, were expressed in most tissues, while AetCCT15,AetCCT21 and AetCCT25 were expressed in seeds, respectively. The AetCCT family could respond to different exogenous hormones. After 24 hours and 72 hours of hormone application, all members showed the same response to the hormone, but different members had different responses to different hormones. The results indicated that the family members had some diversity in function and exercise mode, and might participate in different growth and development processes. Light condition affected the expression of AetCCT, indicating that the effect of light and vernalization is an important factor to affect and regulate the gene expression of the family. The results are helpful to explore the rules of evolution, acclimation and evolution of wheat (T.aestivum), and to understand the formation and interaction network of important agronomic traits.
【作者单位】：山西省农业科学院小麦研究所;山西省农业科学院作物科学研究所作物遗传与分子改良山西省重点实验室;中国科学院合肥物质科学研究院技术生物与农业工程研究所;
【基金】：国家自然科学基金青年基金(No.31601307) 山西省农业攻关(No.20150311001-5) 山西省自然科学基金(No.2016011001) 山西省农业科学院博士基金(No.YBSJJ1503)
【分类号】：Q943.2

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