苹果属无融合生殖SERK1基因的克隆与生物信息学分析

发布时间：2018-10-20 16:42

【摘要】：[目的]为探讨苹果属植物无融合生殖分子机制。[方法]以苹果属平邑甜茶及杂种后代33#为试材,以苹果基因组CDS序列设计引物,通过PCR扩增技术克隆出SERK同源基因的c DNA全长序列,命名为Mh SERK1和MhdSERK1(Gen Bank登录号JQ231273和JQ231272),利用实时定量RTq PCR的方法检测了这两个基因在平邑甜茶和杂种后代各组织和器官中的表达模式。[结果]序列分析显示Mh SERK1和Mhd SERK1编码区序列全长为1 899 bp和1 881 bp,分别编码632和626个氨基酸,其氨基酸序列与其他植物的SERK1同源基因所编码的氨基酸同源性都在80%以上,特别是与葡萄科龙眼品种同源性最高,高达92.56%,与模式植物拟南芥、烟草等植物的SERK同源基因都具有很高的同源性。实时定量PCR结果表明,在平邑甜茶和杂种后代不同组织、花器官中SERK1基因的表达量存在差异,其中在子房中的表达量最高,在营养生长的组织中表达量很低,在平邑甜茶花蕾期的子房中表达量最高。[结论]推测该基因在平邑甜茶和杂种后代的生殖发育过程中可能发挥重要作用。
[Abstract]:[objective] to study the molecular mechanism of apomixis in apple. [methods] the full-length c DNA sequence of SERK homologous gene was cloned by PCR amplification with primer designed from apple genomic CDS sequence and its hybrid progeny 32#. Named Mh SERK1 and MhdSERK1 (Gen Bank accession numbers JQ231273 and JQ231272, the expression patterns of these two genes in tissues and organs of sweet tea and hybrid progenies of Pingyi were detected by real-time quantitative RTq PCR. [results] sequence analysis showed that the total length of Mh SERK1 and Mhd SERK1 coding region was 1 899 bp and 1 881 bp, encoding 632 and 626 amino acids, respectively. The amino acid sequence of the amino acid sequence was more than 80% homology with the SERK1 homologous gene of other plants. Especially with grape longan varieties the highest homology up to 92.56 and model plants such as Arabidopsis tobacco and other plants have high homology of SERK homology. The results of real-time quantitative PCR showed that there were differences in the expression of SERK1 gene in different tissues of sweet tea and hybrid progenies in Pingyi, especially in ovary and low expression in vegetative growth tissue. The highest expression level was found in the ovary of the flower bud stage of sweet tea in Pingyi. [conclusion] it is speculated that this gene may play an important role in the reproductive development of sweet tea and hybrid progenies.
【作者单位】：沈阳农业大学林学院;沈阳农业大学园艺学院;辽宁省本溪市桓仁县林业局;
【基金】：国家自然科学基金“苹果属四倍性皱叶矮生株系的生殖特征及形成机理研究”(30971975)
【分类号】：S661.1

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