蓝鹇源H9N2亚型禽流感病毒HA和NA基因序列分析

发布时间：2018-10-30 14:29

【摘要】：为了探查禽流感病毒H9N2在圈养蓝鹇中的流行规律及变异情况,从圈养蓝鹇(Lophura swinhoii)分离1株H9N2亚型禽流感病毒,对其HA及NA基因进行PCR扩增并进行序列分析。本分离株的HA基因序列与Genbank中广西鸡源2014年分离株A/chicken/Guangxi/CLB02/2014(H9N2)(KT023046)的核苷酸序列相似性最高,为99%;而NA基因与登录号为KM455874,2013人源分离株A/Lengshuitan/11197/2013(H9N2)的NA核苷酸序列相似性最高,为97%。HA蛋白序列分析显示:HA的226位氨基酸残基为亮氨酸(Leu),具有哺乳动物SAα2-6受体结合的特性,裂解位点处的基序PSRSSR↓GL,明显发生1个碱性氨基酸的变异。HA蛋白具有9个潜在N-糖基化位点,分别位于29、82、141、218、298、305、313、492、551位;HA受体结合位点氨基酸变异明显。NA蛋白序列分析显示:NA蛋白颈部63~65位氨基酸缺失,具有7个潜在的糖基化位点,分别位于69、86、146、200、234、264、368位;NA蛋白红细胞结合位点、抗原决定簇区域氨基酸变异活跃。HA、NA基因进化树分析显示该分离株属于欧亚分支中Y280-Like小进化分支,与疫苗株虽属于同一进化分支中,但亲缘关系较远。蓝鹇源H9N2亚型禽流感病毒(LS/GD/P29/16)HA和NA基因发生一定程度的变异,需要加强流行病学监测。
[Abstract]:In order to investigate the prevalence and variation of avian influenza virus (H9N2) in captivity blue pheasant, a strain of H9N2 subtype avian influenza virus was isolated from (Lophura swinhoii) of captive blue pheasant. Its HA and NA genes were amplified by PCR and sequenced. The nucleotide sequence of A/chicken/Guangxi/CLB02/2014 (H9N2) (KT023046) from Guangxi chicken in 2014 was the most similar between the HA gene sequence of this isolate and that of A/chicken/Guangxi/CLB02/2014 (H9N2) (KT023046) in Genbank. The nucleotide sequence similarity between NA gene and A/Lengshuitan/11197/2013 (H9N2) was the highest. The nucleotide sequence analysis of 97%.HA protein showed that the 226-amino acid residue of HA was leucine (Leu),. SA 伪 2-6 receptor binding was observed in mammals, and one basic amino acid was found in the PSRSSR GL, at the cleavage site. The HA protein had nine potential N-glycosylation sites, located at position 29821418298305313492551, respectively. The amino acid variation of HA receptor binding site was obvious. NA protein sequence analysis showed that there were 7 potential glycosylation sites in the neck of NA protein, which were located at 69-86146200234264368; The HA,NA gene phylogenetic tree analysis showed that the isolated strain belonged to the small evolutionary branch of Y280-Like in Eurasia branch and belonged to the same evolutionary branch as the vaccine strain. But the kinship is far away. The HA and NA genes of H9N2 subtype avian influenza virus (LS/GD/P29/16) from the blue pheasant are mutated to some extent, so it is necessary to strengthen epidemiological surveillance.
【作者单位】：广州动物园;华南农业大学;
【分类号】：S852.65

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