芍药查尔酮异构酶基因CHI的表达特性分析

发布时间：2018-11-04 11:45

【摘要】：为了揭示查尔酮异构酶基因(chalcone isomerase gene,CHI)在芍药花瓣中的表达规律与特点,以不同芍药托桂型品种(‘金辉’‘彤云金焰’‘红楼锦菊’)4个不同发育时期(花蕾期、初开期、盛开期、衰败期)中的内、外花瓣为对象,用qPCR分别检测CHI基因的表达水平,分析其在不同芍药品种、不同发育时期以及内外花瓣之间的表达差异。结果显示:不同发育时期同一品种芍药花瓣组织CHI基因的表达量存在一定差异,从花蕾期到衰败期整体表现为上升趋势;不同品种同一发育时期‘彤云金焰’花瓣组织(内瓣和外瓣)CHI基因的表达水平明显高于‘金辉’和‘红楼锦菊’的;同一芍药品种在相同发育时期内瓣组织的CHI基因表达量均明显高于外瓣组织的。鉴于不同芍药品种、不同发育时期内外花瓣间颜色存在差异,推测CHI基因表达量可能与芍药花瓣颜色的形成有关。该结果可为深入研究芍药CHI功能并开展芍药花色遗传改良分子育种研究提供技术支撑。
[Abstract]:In order to reveal the expression rule and characteristics of chalcone isomerase gene (chalcone isomerase gene,CHI in petal of Paeonia lactiflora, four different developmental stages (flower bud stage) of different petal of peony ('Jinhui', 'Tongyun Jin Yan' Honglou Jinju') were used. QPCR was used to detect the expression level of CHI gene, and to analyze the expression difference of CHI gene in different peony varieties, different developmental stages and between inner and outer petals of Paeonia lactiflora. The results showed that the expression of CHI gene in petal tissues of the same variety of Paeonia lactiflora was different in different developmental stages. The expression level of CHI gene in petals (inner and outer valves) of 'Tong Yun Jin Yan' was significantly higher than that of 'Jinhui' and 'Honglou Jinju' at the same developmental stage of different varieties. The expression of CHI gene in the same peony floral tissue at the same developmental stage was significantly higher than that in the outer flap tissue. In view of the difference of petal color between petal and petal at different developmental stages, it is speculated that the expression of CHI gene may be related to the formation of petal color in Paeonia lactiflora. The results can provide technical support for further study on the CHI function of Paeonia lactiflora and molecular breeding for genetic improvement of the flower color of Paeonia lactiflora.
【作者单位】：扬州大学动物科学与技术学院;扬州大学园艺与植物保护学院;
【基金】：国家自然科学基金(31372097,31400592) 江苏省高校自然科学研究重大项目(13KJA210005)
【分类号】：S682.12

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