肥胖型和瘦肉型猪的脂肪、肝脏及骨骼肌组织中CIDE家族基因表达水平的比较研究（英文）

发布时间：2018-11-07 21:42

【摘要】：目的:研究CIDE家族基因在肥胖型和瘦肉型猪的脂肪、肝脏及肌肉组织中的基因表达水平差异,并初步探CIDE家族基因与脂质代谢的关系。创新点:首次在肥胖型与瘦肉型猪模型中解释CIDE家族基因可以调节脂质代谢,并有助于脂肪沉积及导致肥胖。方法:采用荧光定量聚合酶链式反应(qP CR)检测肥胖型蓝塘猪和瘦肉型杜长大猪的脂肪、肝脏和骨骼肌中CIDE家族基因、SREBP-1c、PGC-1α、HNF-4α、FASN、DGAT1和DGAT2、perlipin 2等基因表达水平。采用血浆生化指标仪试剂盒检测两个品种猪血浆中甘油三酯、葡萄糖、游离脂肪酸及胆固醇的含量。结论:肥胖型蓝塘猪脂肪和背最长肌组织中的Cidea和Cidec,及肝脏中Cidec的基因表达量明显高于瘦肉型杜长大猪。在脂肪组织中,脂质代谢相关的基因(包括SREBP-1c、PGC-1α、HNF-4α、FASN、DGAT1和DGAT2基因)表达量都是蓝塘猪高于杜长大猪。蓝塘猪肝脏中的SREBP-1c、HNF-4α和PGC-1α基因表达水平显著高于杜长大猪。蓝塘猪背最长肌组织的SREBP-1c、HNF-4α、PGC-1α和DGAT2基因表达量高于杜长大猪。然而,蓝塘猪的脂肪、肝脏及背最长肌三种组织中的perlipin 2的表达量显著低于杜长大猪。此外,蓝塘猪血浆中的甘油三酯、葡萄糖及游离脂肪酸浓度明显高于杜长大猪。通过相关性分析,我们发现肥胖型和瘦肉型猪不同组织中的CIDE家族基因表达水平与背部脂肪厚度、腹部脂肪重量、血浆中的甘油三酯、葡萄糖及游离脂肪酸浓度有明显的正向相关性。综上所述,CIDE家族基因可以调节脂质代谢,并促进脂肪沉积及导致肥胖。
[Abstract]:Aim: to study the difference of gene expression of CIDE family gene in fat, liver and muscle tissues of obese pigs and lean pigs, and to explore the relationship between CIDE family genes and lipid metabolism. Innovation: for the first time, CIDE family genes can regulate lipid metabolism, contribute to fat deposition and lead to obesity in obese and lean pig models. Methods: fluorescence quantitative polymerase chain reaction (qP CR) was used to detect CIDE family genes, SREBP-1c,PGC-1 伪, HNF-4 伪, FASN,DGAT1 and DGAT2, in fat, liver and skeletal muscle of obese and lean Landrace pigs. Perlipin 2 gene expression level. The contents of triglycerides, glucose, free fatty acids and cholesterol in plasma of two breeds of pigs were determined by plasma biochemical indicator kit. Conclusion: the expression of Cidea and Cidec, in fat and longissimus dorsi muscle tissues and Cidec gene expression in liver of obese blue tang pigs were significantly higher than those of lean Duroc pigs. In adipose tissue, the expression levels of genes related to lipid metabolism (including SREBP-1c,PGC-1 伪, HNF-4 伪, FASN,DGAT1 and DGAT2 genes) were higher in Lantang pigs than in du grown-up pigs. The expression levels of SREBP-1c,HNF-4 伪 and PGC-1 伪 in the liver of Lantang pig were significantly higher than those in Du-grown pig. The expression of SREBP-1c,HNF-4 伪, PGC-1 伪 and DGAT2 genes in the longissimus dorsi muscle tissue of Lantang pig was higher than that in Du-grown pig. However, the expression of perlipin 2 in the fat, liver and longissimus dorsi of Lantang pigs was significantly lower than that of Duroc pigs. In addition, the concentrations of triglycerides, glucose and free fatty acids in the plasma of Lantang pigs were significantly higher than those in Duroc pigs. By correlation analysis, we found that the expression level of CIDE family genes in different tissues of obese and lean pigs was related to the thickness of back fat, weight of abdominal fat, triglyceride in plasma. The concentrations of glucose and free fatty acids were positively correlated. In conclusion, CIDE family genes regulate lipid metabolism, promote fat deposition and lead to obesity.
【作者单位】： Ministry
【基金】：supported by the National Natural Science Foundation of China(No.31402086) the China Agriculture Research System(CARS-36) the National Basic Research Program(973)of China(Nos.2013CB127301 and 2013CB127304) the Guangdong International Science and Technology Cooperation Program(No.2014A050503049) the Science and Technology Program of Guangdong Province(No.2016A020210041),China
【分类号】：S828

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