亚油酸对奶牛乳腺上皮细胞乳脂肪和乳蛋白合成相关基因表达的影响

发布时间：2018-11-08 09:16

【摘要】：该文主要研究了添加不同浓度的亚油酸(顺-9,顺-12-十八碳二烯酸)(0、20、40、80、120μmol/L)对奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMECs)乳脂和乳蛋白合成相关基因表达的影响。通过MTT法检测细胞活力,利用甘油三酯试剂盒检测BMECs内甘油三酯(triglyceride,TAG)的合成量,实时荧光定量PCR检测乳脂和乳蛋白合成相关基因的表达。结果表明:(1)添加20μmol/L的亚油酸能促进BMECs的增殖,120μmol/L的亚油酸组BMECs的相对增殖率显著低于对照组(P0.05);(2)40μmol/L和80μmol/L亚油酸添加组BMECs中TAG的合成量显著高于对照组(P0.05);(3)添加20μmol/L亚油酸显著上调αs1-酪蛋白(casein alpha s1 identifiers symbols,CSN1S1)和κ-酪蛋白(κ-casein,CSN3)基因的表达,而80μmol/L和120μmol/L亚油酸显著下调CSN1S1和CSN3基因的表达(P0.05);(4)120μmol/L亚油酸上调了真核启始4E结合蛋白-1(eukaryotic initiation factor 4E-binding-protein-1,4EBP1)基因的表达,下调了核糖体蛋白S6激酶-1(ribosomal protein S6 kinase-1,RPS6K1)基因的表达(P0.05);(5)添加20~120μmol/L亚油酸显著下调脂肪酸合酶(fatty acid synthase,FASN)、硬脂酰辅酶A去饱和酶(stearoyl-Co A desaturase,SCD)、脂肪酸结合蛋白-3(fatty acid-binding protein-3,FABP3)的基因表达(P0.05);(6)20μmol/L亚油酸添加组过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptorγ,PPARG)基因表达量显著高于对照组,而120μmol/L亚油酸组PPARG、固醇调节元件结合因子-1(sterol regulatory element binding factor-1,SREBF1)的表达量显著低于对照组(P0.05)。综上所述,20~40μmol/L的亚油酸对BMECs乳脂肪和乳蛋白合成有较好的促进效果。
[Abstract]:The effects of different concentrations of linoleic acid (cis-9, cis-12-octadecadienoic acid) on the expression of genes related to milk fat and milk protein synthesis in dairy cow mammary epithelial cells (bovine mammary epithelial cells,BMECs) were studied. MTT assay was used to detect cell viability, triglyceride kit was used to detect the synthesis of triglyceride (triglyceride,TAG) in BMECs, and real-time quantitative PCR was used to detect the expression of genes related to milk fat and milk protein synthesis. The results showed that: (1) 20 渭 mol/L linoleic acid could promote the proliferation of BMECs, and the relative proliferation rate of BMECs in the linoleic acid group of 120 渭 mol/L was significantly lower than that in the control group (P0.05); (2) the synthesis of TAG in 40 渭 mol/L and 80 渭 mol/L linoleic acid group was significantly higher than that in the control group (P0.05). (3) 20 渭 mol/L linoleic acid significantly up-regulated the expression of 伪 s1-casein (casein alpha S1 identifiers symbols,CSN1S1 and 魏 -casein (魏 -casein,CSN3), while 80 渭 mol/L and 120 渭 mol/L linoleic acid significantly down-regulated the expression of CSN1S1 and CSN3 genes (P0.05). (4) 120 渭 mol/L linoleic acid up-regulated the expression of eukaryotic initiation 4e binding protein-1 (eukaryotic initiation factor 4E-binding-protein-1binding-protein-4EBP1 gene and down-regulated the expression of ribosomal protein S6 kinase 1 (ribosomal protein S6 kinase-1,RPS6K1 (P0.05). (5) 20 渭 mol/L linoleic acid significantly down-regulated fatty acid synthase (fatty acid synthase,FASN), stearyl coenzyme A desaturase (stearoyl-Co A desaturase,SCD), fatty acid binding protein-3 (fatty acid-binding protein-3,). FABP3) gene expression (P0.05); (6) the expression of peroxisome proliferator activated receptor (peroxisome proliferator-activated receptor 纬, PPARG) gene in 20 渭 mol/L linoleic acid group was significantly higher than that in control group, while PPARG, in 120 渭 mol/L linoleic acid group was significantly higher than that in control group. The expression of steroid regulatory element binding factor-1 (sterol regulatory element binding factor-1,SREBF1 was significantly lower than that of control group (P0.05). In conclusion, 20 ~ 40 渭 mol/L linoleic acid has a good effect on the synthesis of fat and protein in BMECs milk.
【作者单位】：内蒙古农业大学动物科学学院;
【基金】：内蒙古自治区高等学校“青年科技英才支持计划”(批准号: NJYT-14-B05)资助的课题;国家自然科学基金（批准号：31360559）
【分类号】：S823

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