PLA3基因在水稻种子萌发及幼苗生长中的作用研究
发布时间：2018-11-17 10:31
【摘要】：水稻是我国主要的粮食作物。植物激素ABA与种子萌发关系密切,是影响种子萌发的主要激素之一。水稻.PLASTOCHRON是一种叶原基形成间隔期调控基因,并与植株由营养生长过渡到生殖生长相关。前人研究结果表明PLA3突变体(pla3)种子在萌发过程中对ABA不敏感。为进一步探讨PLA3在水稻种子萌发及幼苗生长过程中的作用及其与ABA的互作关系,本研究以野生型水稻、pla3突变体、ABA不敏感突变体abll、水稻冈46B和D84B等为研究材料,分别研究了PLA3基因对水稻种子萌发及幼苗生长的影响、PLA3与ABA的相互关系以及PLA3对杂交水稻制种过程中穗发芽形成的影响。结果如下：1、pla3突变体筛选及表型观察：体视显微镜下观察发现突变体pla3种子胚较野生型明显偏大,以此表型初步筛选出突变体种子,再通过基因型鉴定验证；pla3种子易于萌发、但其幼苗期叶片畸形、生长速度慢、植株矮化且分蘖数增加,且不能进行生殖生长,完全不育。2、PLA3基因参与水稻种子萌发及幼苗生长过程的调控且受ABA的诱导。对冈46B和D84B种子萌发性状比较发现,36h时冈46B的萌发率为90%,易萌发；D84B的萌发率为70%,难萌发。分别对冈46B和D84B种子萌发及幼苗生长中PLA3的表达量分析发现,D84B中PLA3的表达量显著高于冈46B。ABA (CK、2μmol/L和5 μmol/L)处理两种水稻发现,与CK相比,2μmol/L和5μmol/L ABA处理下激素含量和淀粉酶的活性降低,PLA3的表达量上升：ABA不敏感突变体abll中PLA3基因的表达量显著低于野生型,初步表明PLA3与ABA的信号转导途径相关。3、PLA3基因影响杂交水稻穗发芽的形成。分别以R9311和R416为父本、以Y58S和Ⅱ-32A为母本制种得到穗发芽有明显差异的杂交组合Y两优1号(易穗发芽)和Ⅱ优416(难穗发芽)；分别对Y两优1号和Ⅱ优416种子成熟过程中主要激素ZR、GA1、 ABA、IAA含量的动态变化、PLA3表达量进行了分析。结果表明,Y两优1号内源激素ZR、GA1、IAA都高于Ⅱ优416,ABA含量则相反；Ⅱ优416中PLA3基因的表达量更高。推测PLA3基因在此过程中可能是通过ABA诱导其基因表达上调而发挥作用。本论文相关试验探讨了水稻PLA3基因在水稻种子萌发及幼苗生长中的作用。结果表明PLA3在水稻种子萌发过程中可能参与ABA的信号转导,且与水稻穗发芽相关。为研究PLA3基因的功能及其参与水稻种子萌发和幼苗生长以及穗发芽的分子机制奠定基础。
[Abstract]:Rice is the main food crop in China. Plant hormone ABA is closely related to seed germination and is one of the main hormones affecting seed germination. Rice. PLASTOCHRON is a regulating gene of leaf primordium formation interval and is related to plant growth transition from vegetative growth to reproductive growth. Previous studies showed that PLA3 mutant (pla3) seeds were insensitive to ABA during germination. In order to further study the role of PLA3 in seed germination and seedling growth of rice and its interaction with ABA, wild type rice, pla3 mutants, ABA insensitive mutants abll, Gang46B and D84B were used as materials. The effects of PLA3 gene on seed germination and seedling growth, the relationship between PLA3 and ABA, and the effect of PLA3 on the formation of spike germination in hybrid rice seed production were studied. The results were as follows: 1 screening and phenotypic observation of the mutant: under stereomicroscopy, the embryo of the mutant pla3 seed was obviously larger than that of the wild type, and the mutant seeds were preliminarily screened with this phenotype and verified by genotypic identification. Pla3 seeds are easy to germinate, but their leaves are malformed at seedling stage, the growth rate is slow, the plants are short, the number of tillers is increased, and they can not reproduce and grow, and they are completely sterile. PLA3 gene is involved in the regulation of rice seed germination and seedling growth and is induced by ABA. The comparison of germination characters between Gang46B and D84B showed that the germination rate of Gang46B was 90 and easy to germinate at 36h, and the germination rate of D84B was 70, which was difficult to germinate. The expression of PLA3 in seed germination and seedling growth of Gang46B and D84B were analyzed respectively. The results showed that the expression of PLA3 in D84B was significantly higher than that in CK,2 渭 mol/L and 5 渭 mol/L treated with Gang46B (CK,2 渭 mol/L and 5 渭 mol/L), compared with CK. Under the treatment of 2 渭 mol/L and 5 渭 mol/L ABA, the hormone content and amylase activity decreased, and the expression of PLA3 increased. The expression of PLA3 gene in ABA insensitive mutant abll was significantly lower than that in wild type, which indicated that PLA3 was related to ABA signal transduction pathway. PLA3 gene affects the formation of hybrid rice panicle germination. Using R9311 and R416 as male parents and Y58S and 鈪，
本文编号：2337460
[Abstract]:Rice is the main food crop in China. Plant hormone ABA is closely related to seed germination and is one of the main hormones affecting seed germination. Rice. PLASTOCHRON is a regulating gene of leaf primordium formation interval and is related to plant growth transition from vegetative growth to reproductive growth. Previous studies showed that PLA3 mutant (pla3) seeds were insensitive to ABA during germination. In order to further study the role of PLA3 in seed germination and seedling growth of rice and its interaction with ABA, wild type rice, pla3 mutants, ABA insensitive mutants abll, Gang46B and D84B were used as materials. The effects of PLA3 gene on seed germination and seedling growth, the relationship between PLA3 and ABA, and the effect of PLA3 on the formation of spike germination in hybrid rice seed production were studied. The results were as follows: 1 screening and phenotypic observation of the mutant: under stereomicroscopy, the embryo of the mutant pla3 seed was obviously larger than that of the wild type, and the mutant seeds were preliminarily screened with this phenotype and verified by genotypic identification. Pla3 seeds are easy to germinate, but their leaves are malformed at seedling stage, the growth rate is slow, the plants are short, the number of tillers is increased, and they can not reproduce and grow, and they are completely sterile. PLA3 gene is involved in the regulation of rice seed germination and seedling growth and is induced by ABA. The comparison of germination characters between Gang46B and D84B showed that the germination rate of Gang46B was 90 and easy to germinate at 36h, and the germination rate of D84B was 70, which was difficult to germinate. The expression of PLA3 in seed germination and seedling growth of Gang46B and D84B were analyzed respectively. The results showed that the expression of PLA3 in D84B was significantly higher than that in CK,2 渭 mol/L and 5 渭 mol/L treated with Gang46B (CK,2 渭 mol/L and 5 渭 mol/L), compared with CK. Under the treatment of 2 渭 mol/L and 5 渭 mol/L ABA, the hormone content and amylase activity decreased, and the expression of PLA3 increased. The expression of PLA3 gene in ABA insensitive mutant abll was significantly lower than that in wild type, which indicated that PLA3 was related to ABA signal transduction pathway. PLA3 gene affects the formation of hybrid rice panicle germination. Using R9311 and R416 as male parents and Y58S and 鈪，
本文编号：2337460
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