大豆ms1轮回群体应用于转EPSPS基因大豆育种改良研究

发布时间：2018-12-18 13:40

【摘要】：针对抗草甘膦转EPSPS基因大豆遗传基础狭窄的问题,利用ms1轮回群体拓宽抗草甘膦转基因大豆的遗传基础。利用遗传基础丰富的ms1基础群体,与现有的抗草甘膦转EPSPS基因大豆新品系冀K32、冀K331、冀K69、冀K964建立抗草甘膦C0群体,C0群体改良后形成C1群体。分析基础、C0、C1 3个群体的品质、产量等农艺性状。结果表明,C1群体蛋白变化为35.66%~48.02%;脂肪变化为16.34%~22.92%;株高变化为46.00~158.00 cm;分枝数变化为0~8个;百粒质量变化为10.30~25.30 g;单株重变化为1.60~59.60 g。C1群体中不育株所占比例为19.20%,抗草甘膦性株所占比例为70.54%。利用大豆ms1基因构建了一个含EPSPS基因的转基因大豆轮回群体,该群体性状分离广泛,遗传多样性丰富,适合转基因大豆育种材料筛选。
[Abstract]:Aiming at the narrow genetic basis of glyphosate resistant soybean transgenic with EPSPS gene, the genetic basis of glyphosate resistant soybean was broadened by ms1 cycle population. The populations resistant to glyphosate C0 were established by using the ms1 basic population with abundant genetic basis and the existing lines of transgenic soybean resistant to glyphosate, Ji K32, Ji K331, Ji K69 and Ji K964, and the C _ 1 population was improved to form a C1 population. On the basis of analysis, the agronomic characters of C _ 0 C _ 1 population, such as quality, yield and so on, were analyzed. The results showed that the changes of C1 population protein were 35.66 and 48.02, the changes of fat were 16.34 and 22.922, the changes of plant height were 46.00 and 158.00 cm; branches were 0 ~ 8, the weight of 100 seeds were 10.30 and 25.30 g. The percentage of sterile plants and glyphosate resistant plants was 19.20 and 70.54 in 1.60,59.60 g.C1 population, respectively. A transgenic soybean recurrent population containing EPSPS gene was constructed by using soybean ms1 gene. This population was characterized by extensive segregation and rich genetic diversity, which was suitable for selection of transgenic soybean breeding materials.
【作者单位】：河北省农林科学院粮油作物研究所河北省作物遗传育种实验室国家大豆中心石家庄分中心农业部黄淮海大豆生物学与遗传育种重点实验室;
【基金】：转基因生物新品种培育重大专项(2016ZX08004-001;2014ZX0800402B) 产业技术体系建设项目(CARS-004-PS06)
【分类号】：S565.1

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