山羊FGF21基因克隆及其在肌内脂肪细胞中的表达模式研究
发布时间：2018-12-18 17:13
【摘要】：本研究旨在获得山羊FGF21基因序列,阐明其组织表达特性,同时检测FGF21基因与FGF受体(FGFR)在山羊肌内前体脂肪细胞分化过程中的表达水平。以简州大耳羊为试验动物,采用II型胶原酶消化获得山羊原代肌内前体脂肪细胞,采用RT-PCR技术克隆山羊FGF21基因,利用荧光定量PCR(qPCR)技术检测FGF21基因在成年山羊不同组织中的表达特性及FGF21与FGF受体在山羊肌内前体脂肪细胞分化过程中的表达差异。结果,克隆获得山羊FGF21基因(GenBank登录号:KT288195)全长序列666bp,其中开放阅读框630bp,编码209个氨基酸。FGF21mRNA在山羊心、肝、脾、肺、肾、脂肪和背最长肌中均检测到表达,且在肝和脂肪中存在较高水平表达。FGF21基因与FGFR1、FGFR2表达模式相同,均是在诱导分化第2天的肌内脂肪细胞中表达水平最高,极显著高于其他天数(P0.01)。FGF21基因在山羊脂肪组织中存在较高水平的表达,并且在诱导分化的肌内前体脂肪细胞成脂分化第2天表达水平最高,推测其可能通过受体FGFR1或FGFR2在山羊肌内前体脂肪细胞分化早期发挥调控作用,此研究结果为进一步揭示FGF21基因在山羊肌内脂肪沉积中的作用机制提供了数据支持。
[Abstract]:The aim of this study was to obtain the goat FGF21 gene sequence and to elucidate the expression characteristics of goat FGF21 gene, and to detect the expression level of FGF21 gene and FGF receptor (FGFR) during the differentiation of goat intramuscular precursor adipocytes. Goat intramuscular preadipocytes were obtained by II collagenase digestion and RT-PCR technique was used to clone goat FGF21 gene. The expression characteristics of FGF21 gene in different tissues of adult goat and the difference of expression of FGF21 and FGF receptor in the differentiation of goat intramuscular precursor adipocytes were detected by fluorescence quantitative PCR (qPCR) technique. Results the full-length sequence of goat FGF21 gene (GenBank accession number: KT288195) was 666 BP. The open reading frame 630 BP encoded 209 amino acids. The expression of FGF21mRNA was detected in heart, liver, spleen, lung, kidney, fat and longissimus dorsi muscle. The expression pattern of FGF21 gene was the same as that of FGFR1,FGFR2, and the expression level of FGF21 gene was the highest in intramuscular adipocytes on the second day of differentiation. The expression of FGF21 gene in adipose tissue of goat was higher than that in other days (P0.01), and the expression level of FGF21 gene was the highest on the second day of adipogenic differentiation of intramuscular precursor adipocytes induced by differentiation. It is speculated that it may play a regulatory role in the early differentiation of goat intramuscular precursor adipocytes by receptor FGFR1 or FGFR2. The results provide data support for further revealing the mechanism of the role of FGF21 gene in goat intramuscular fat deposition.
【作者单位】： 西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：四川省“十三五”畜禽育种攻关项目(2016NYZ0045) 四川省科技创新产业链示范工程重大项目(2014NZ0003) 中央高校基本科研业务费专项资金项目(2016NZYQN36)
【分类号】：S827
本文编号：2386204
[Abstract]:The aim of this study was to obtain the goat FGF21 gene sequence and to elucidate the expression characteristics of goat FGF21 gene, and to detect the expression level of FGF21 gene and FGF receptor (FGFR) during the differentiation of goat intramuscular precursor adipocytes. Goat intramuscular preadipocytes were obtained by II collagenase digestion and RT-PCR technique was used to clone goat FGF21 gene. The expression characteristics of FGF21 gene in different tissues of adult goat and the difference of expression of FGF21 and FGF receptor in the differentiation of goat intramuscular precursor adipocytes were detected by fluorescence quantitative PCR (qPCR) technique. Results the full-length sequence of goat FGF21 gene (GenBank accession number: KT288195) was 666 BP. The open reading frame 630 BP encoded 209 amino acids. The expression of FGF21mRNA was detected in heart, liver, spleen, lung, kidney, fat and longissimus dorsi muscle. The expression pattern of FGF21 gene was the same as that of FGFR1,FGFR2, and the expression level of FGF21 gene was the highest in intramuscular adipocytes on the second day of differentiation. The expression of FGF21 gene in adipose tissue of goat was higher than that in other days (P0.01), and the expression level of FGF21 gene was the highest on the second day of adipogenic differentiation of intramuscular precursor adipocytes induced by differentiation. It is speculated that it may play a regulatory role in the early differentiation of goat intramuscular precursor adipocytes by receptor FGFR1 or FGFR2. The results provide data support for further revealing the mechanism of the role of FGF21 gene in goat intramuscular fat deposition.
【作者单位】： 西南民族大学生命科学与技术学院;青藏高原动物遗传资源保护与利用四川省重点实验室;
【基金】：四川省“十三五”畜禽育种攻关项目(2016NYZ0045) 四川省科技创新产业链示范工程重大项目(2014NZ0003) 中央高校基本科研业务费专项资金项目(2016NZYQN36)
【分类号】：S827
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