葡萄抗白粉病相关基因γVPE的克隆与表达分析
发布时间：2018-12-18 18:15
【摘要】：该研究采用RT-PCR技术,从抗病的中国野生华东葡萄‘白河-35-1’和感病的欧洲葡萄‘佳丽酿’中克隆了液泡加工酶基因(γVPE),分别命名为VpγVPE和VvCγVPE。克隆的2个γVPE基因cDNA长度均为1 624bp,ORF为1 482bp,编码493个氨基酸。氨基酸多序列对比分析发现,‘白河-35-1’、‘佳丽酿’、‘无核白’和‘黑比诺’葡萄中的γVPE基因底物结合口袋域的3个关键氨基酸之一的丝氨酸(Ser395)均变为丙氨酸(Ala),与其他植物的VPE基因底物口袋结合域有所不同。实时荧光定量PCR表明,在白粉菌诱导后的不同时期内,γVPE基因在感病葡萄和抗病葡萄中的表达模式不同,抗病株系中VpγVPE基因的表达量在诱导后的前期(4h和48h)和后期(168h)均有所增加,而感病株系中VvCγVPE基因在诱导后4h表达量最高,随后降低。γVPE基因在白粉菌诱导后不同时期内表达量的变化,表明γVPE基因在一定程度上与葡萄的抗性相关。研究结果为进一步揭示γVPE基因在抗病过程中的分子机理奠定了基础。
[Abstract]:Using RT-PCR technique, the vacuolar processing enzyme gene (纬 VPE),) was cloned from the disease-resistant wild Chinese grape 'Baihe-35-1' and the susceptible European grape 'Jiali'. 纬 VPE), was named Vp 纬 VPE and VvC 纬 VPE., respectively. The cDNA length of the two 纬 VPE genes was 1 624 BP, and the ORF was 1 482 BP, encoding 493 amino acids. Comparative analysis of amino acid sequences revealed that 'Baihe-35-1', 'Jiali', The serine (Ser395), one of the three key amino acids in the substrate binding pocket domain of 纬 VPE gene in 'denuclearized white' and 'Pinot Noir' grapes, is different from the VPE gene substrate pocket binding domain in other plants. Real-time fluorescence quantitative PCR showed that the expression patterns of 纬 VPE gene in susceptible and disease-resistant grapes were different at different stages after induction by powdery mildew. The expression of Vp 纬 VPE gene increased in the early stage (4 h and 48 h) and later stage (168 h) of resistant lines, but the expression of VvC 纬 VPE gene was the highest in susceptible lines at 4 h after induction. The changes of 纬 VPE gene expression in different period after induced by powdery mildew indicated that 纬 VPE gene was related to grape resistance to some extent. The results laid a foundation for further revealing the molecular mechanism of 纬 VPE gene in the process of disease resistance.
【作者单位】： 西北农林科技大学园艺学院旱区作物逆境生物学国家重点实验室农业部西北地区园艺作物生物学与种质创制重点实验室;
【基金】：国家自然科学基金(31171926) 国家现代农业产业技术体系建设专项(CARS-30-yz-7) 杨凌示范区农业科技示范推广项目(2015-TS-10)
【分类号】：S436.631
，
本文编号：2386285
[Abstract]:Using RT-PCR technique, the vacuolar processing enzyme gene (纬 VPE),) was cloned from the disease-resistant wild Chinese grape 'Baihe-35-1' and the susceptible European grape 'Jiali'. 纬 VPE), was named Vp 纬 VPE and VvC 纬 VPE., respectively. The cDNA length of the two 纬 VPE genes was 1 624 BP, and the ORF was 1 482 BP, encoding 493 amino acids. Comparative analysis of amino acid sequences revealed that 'Baihe-35-1', 'Jiali', The serine (Ser395), one of the three key amino acids in the substrate binding pocket domain of 纬 VPE gene in 'denuclearized white' and 'Pinot Noir' grapes, is different from the VPE gene substrate pocket binding domain in other plants. Real-time fluorescence quantitative PCR showed that the expression patterns of 纬 VPE gene in susceptible and disease-resistant grapes were different at different stages after induction by powdery mildew. The expression of Vp 纬 VPE gene increased in the early stage (4 h and 48 h) and later stage (168 h) of resistant lines, but the expression of VvC 纬 VPE gene was the highest in susceptible lines at 4 h after induction. The changes of 纬 VPE gene expression in different period after induced by powdery mildew indicated that 纬 VPE gene was related to grape resistance to some extent. The results laid a foundation for further revealing the molecular mechanism of 纬 VPE gene in the process of disease resistance.
【作者单位】： 西北农林科技大学园艺学院旱区作物逆境生物学国家重点实验室农业部西北地区园艺作物生物学与种质创制重点实验室;
【基金】：国家自然科学基金(31171926) 国家现代农业产业技术体系建设专项(CARS-30-yz-7) 杨凌示范区农业科技示范推广项目(2015-TS-10)
【分类号】：S436.631
，
本文编号：2386285
本文链接：https://www.wllwen.com/kejilunwen/jiyingongcheng/2386285.html
最近更新
教材专著
