花生蔗糖转运蛋白基因AhSUT1的克隆及其在非生物胁迫下的表达分析

发布时间：2019-01-11 20:02

【摘要】：低温、高盐、干旱等非生物胁迫严重影响花生(Arachis hypogaea)生长和产量,而对花生非生物胁迫的研究及抗性基因的挖掘较少。蔗糖转运蛋白(SUC或SUT)影响蔗糖的运输方向、速率和分配,其基因表达受多种因子的调控,包括内源激素、生物逆境和非生物胁迫等。本研究以花生品种‘花育33号’为试验材料,根据cDNA文库中已知的SUT基因全长序列设计引物,通过反转录PCR(RT-PCR)克隆到该基因,命名为AhSUT1。AhSUT1全长为1 861 bp,开放阅读框为1 563 bp,编码521个氨基酸。预测该基因编码的蛋白含有MFS保守结构域,可能定位于细胞质膜上。蛋白序列比对和进化树分析表明,花生与大豆(Glycine max)、蒺藜苜蓿(Medicago truncatula)、鹰嘴豆(Cicer arietinum)、豌豆(Pisum sativum)等豆科植物中的SUT序列相似性最高,亲缘关系最近。荧光定量PCR结果显示,AhSUT1在花生根中的表达受高盐胁迫特异诱导,说明该基因可能参与了花生对高盐胁迫的适应性调控;AhSUT1在花生根中受脱落酸(ABA)诱导,说明该基因对花生盐胁迫的调控可能是以依赖ABA的方式进行的。
[Abstract]:Low temperature, high salt, drought and other abiotic stresses seriously affected the growth and yield of peanut (Arachis hypogaea), but the research on peanut abiotic stress and the digging of resistant genes were few. Sucrose transporter (SUC or SUT) affects the transport direction, rate and distribution of sucrose. Its gene expression is regulated by a variety of factors, including endogenous hormones, biological stresses and abiotic stresses. In this study, the peanut variety 'Huayu 33' was used as experimental material. Primers were designed according to the known full-length sequence of SUT gene in cDNA library. The gene was cloned by reverse transcription PCR (RT-PCR) and named AhSUT1.AhSUT1 as 1 861 bp,. The open reading frame encodes 521 amino acids for 1 563 bp,. It is predicted that the protein encoded by the gene contains a conserved domain of MFS, which may be located on the cytoplasmic membrane. Protein sequence alignment and phylogenetic tree analysis showed that there was the highest similarity of SUT sequence between peanut and (Medicago truncatula), (Cicer arietinum), pea, (Pisum sativum), etc. The results of fluorescence quantitative PCR showed that the expression of AhSUT1 in peanut roots was specifically induced by high salt stress, which suggested that the gene might be involved in the adaptive regulation of peanut under high salt stress. AhSUT1 was induced by abscisic acid (ABA) in peanut roots, indicating that the regulation of the gene on peanut salt stress may be dependent on ABA.
【作者单位】：山东省花生研究所;
【基金】：山东省农业科学院青年科研基金(2016YQN14) 2014年国家“万人计划”青年拔尖人才项目国家花生产业技术体系项目(CARS-14) 山东省自然科学基金(ZR2014YL011和ZR2014YL012) 国家自然科学基金(31000728和31200211) 山东省农业科学院青年英才培养计划,山东省农业科学院农业科技创新工程项目(CXGC2016B02)~~
【分类号】：Q943.2;S565.2

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