西花蓟马clip丝氨酸蛋白酶基因的鉴定与表达分析

发布时间：2019-06-05 17:19

【摘要】：本研究旨在鉴定西花蓟马的clip丝氨酸蛋白酶(clip-domian serine proteases,Fo CSPs)基因,分析其被球孢白僵菌侵染后的表达模式,为研究clip丝氨酸蛋白酶在西花蓟马先天免疫反应中的作用奠定基础。共鉴定出西花蓟马9个clip丝氨酸蛋白酶基因Fo CSPs,每个Fo CSPs编码的氨基酸序列都在氮端有一个发夹(clip)区域,在碳端有一个具有催化功能的丝氨酸蛋白酶区域,催化区域有3个保守的氨基酸,即组氨酸(His),天冬氨酸(Asp)和色氨酸(Ser)。系统进化树分析表明,Fo CSP215与褐飞虱NITRY19位于同一进化支上,Fo CSP261和Fo CSP236与烟草天蛾Ms POAEO,斯氏按蚊Ag CLIP和金小蜂Nv SP16P聚为一支,其余6个Fo CSPs聚为一支,并与烟草天蛾5个Ms HPs聚成的分支汇合在一起。球孢白僵菌侵染后,成虫和若虫Fo CSPs的相对表达量均为上调表达,但程度不同。对于同一基因,在若虫体内的最高相对表达量高于成虫。总之,Fo CSPs可能参与到西花蓟马应对球孢白僵菌侵染的免疫反应中,是西花蓟马生物防治的潜在靶标。
[Abstract]:The purpose of this study was to identify the clip serine protease (clip-domian serine proteases,Fo CSPs) gene of thrips and analyze its expression pattern after infection with Beauveria bassiana. It lays a foundation for studying the role of clip serine protease in innate immune response of western flower thrips. A total of 9 clip serine protease genes Fo CSPs, were identified. Each Fo CSPs encoded amino acid sequence had a hairpin (clip) region at the nitrogen end and a serine protease region with catalytic function at the carbon end. There are three conserved amino acids in the catalytic region, namely histidine (His), aspartic acid (Asp) and tryptophan (Ser). Phylogenetic tree analysis showed that Fo CSP215 and brown planthopper NITRY19 were located on the same evolutionary branch. Fo CSP261 and Fo CSP236 were aggregated into one branch with Anopheles Ms POAEO, Ag CLIP and Nv SP16P, and the other six Fo CSPs were aggregated into one branch. And converged with the five Ms HPs branches of tobacco moths. After Beauveria bassiana infected by Beauveria bassiana, the relative expression of Fo CSPs in adults and nymph was up-regulated, but the degree was different. For the same gene, the highest relative expression in nymph was higher than that in adult. In conclusion, Fo CSPs may be involved in the immune response of thrips to Beauveria bassiana infection, and it is a potential target for biological control of thrips.
【作者单位】：沈阳农业大学植物保护学院;中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室;丹东出入境检验检疫局;
【基金】：基金项目:国家现代农业产业技术体系(CARS-25B07)
【分类号】：S433

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