粳型优势生态型水稻Rf1a基因位点的检测

发布时间：2019-06-14 10:13

【摘要】：【目的】探明粳型优势生态型水稻中各品种(系)在Rf1a基因位点的差异,为粳型杂种优势群的利用提供基础数据。【方法】利用缺失设计引物Rfa-7F/Rfa-7R在粳稻恢复系中可扩增出957bp的片段,在保持系中可扩增出383bp的片段,对日本粳、台湾粳、韩国粳、巴西稻、美国粳、非洲IRAT粳、华北粳、非洲ITA粳、西北粳、东北粳、欧洲粳11个不同生态类型共60份材料进行PCR扩增检测。【结果】有14份材料是Rf1a/Rf1a基因类型,占所有材料的23%;日本粳、台湾粳、华北粳、西北粳和东北粳的所有材料是rf1a/rf1a基因类型,韩国粳、非洲ITA粳和欧洲粳有小部分材料是Rf1a/Rf1a基因类型,巴西粳、美国粳和非洲IRAT的大部分是Rf1a/Rf1a基因类型。【结论】粳型生态型水稻在Rf1a基因位点的差异,可以用引物Rfa-7F/Rfa-7R检测出不同基因类型,为粳型杂种优势群利用提供依据。
[Abstract]:[Objective] To find out the difference of the two varieties (lines) in the japonica-type dominant ecotype rice in the Rf1a gene, and to provide the basic data for the utilization of the japonica-type heterosis group. [Method] The fragment of 957 bp can be amplified in the restorer line of japonica rice by using the missing design primer Rfa-7F/ Rfa-7R, and a fragment of 383 bp can be amplified in the maintainer line, and the PCR amplification detection is carried out on the total of 60 materials of 11 different ecological types in the Japan, Taiwan, South Korea, Brazil, the United States, the African IRAT, the North China, the African ITA, the northwest, the northeast, the northeast, the European and the 11 different ecological types. [Results] There are 14 materials of Rf1a/ Rf1a gene type, accounting for 23% of all materials; all the materials in Japan, Taiwan, North China, North-West and Northeast China are the rf1a/ rf1a gene types, and the South Korea, the African ITA and the European japonica have the small part of the Rf1a/ Rf1a gene type, and most of the Rf1a/ Rf1a gene types in Brazil, the United States and the African IRAT are the Rf1a/ Rf1a gene types. [Conclusion] The different gene types can be detected by using the primers Rfa-7F/ Rfa-7R to provide the basis for the utilization of the hybrid-type heterosis group.
【作者单位】：宁夏农林科学院农作物研究所;
【基金】：国家自然科学基金项目(31160273) 宁夏自然科学基金项目(NZ14185) 宁夏回族自治区农业育种专项水稻新品种选育项目(2013NYY20305)
【分类号】：S511.22

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