转TfdA基因提高玉米抗除草剂2,4-D的能力

发布时间：2019-06-14 15:54

【摘要】：本研究以玉米(Zea mays)为材料,通过农杆菌(Agrobacterium tumefaciens)介导的茎尖转化法将对除草剂2,4-二氯苯氧乙酸(2,4-D)降解的Tfd A基因转入玉米,筛选出对除草剂2,4-D具有高抗性的转基因玉米植株。研究表明,喷施20 g·L~(-1)的2,4-D溶液7 d后,91%的野生型玉米植株出现根和茎肿胀畸形且横向生长、叶片枯黄脱落等现象,而转基因植株生长良好,未出现明显的受害症状。随2,4-D处理时间的推移,转基因和非转基因玉米植株的叶绿素含量均逐渐下降,但后者的降幅极显著高于前者。二氨基联苯胺(DAB)染色后发现,经2,4-D处理的野生型植株叶片H_2O_2积累显著高于转基因植株,且前者的丙二醛(MDA)含量也显著高于后者,分别为3.34和2.08μmol·g-1(蛋白)。在2,4-D处理3 d后,转基因植株的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性峰值分别为34.88、4.10和31.56 U·mg-1(蛋白),是其野生型的1.65、1.4和1.7倍(差异极显著)。综上结果表明,转Tfd A基因玉米显著提高了对除草剂2,4-D的抗性。
[Abstract]:In this study, the Tfd A gene of 2,4-dichlorophenoxyacetic acid (2,4-D) was transferred to maize by Agrobacterium tumefaciens-mediated stem-point transformation method, and the transgenic corn plants with high resistance to the herbicide 2,4-D were screened. The results showed that after 7 days of spraying of 20 g 路 L ~ (-1) 2,4-D solution,91% of the wild-type maize plants had the phenomena of root and stem swelling, lateral growth, and shedding of the leaves. The chlorophyll content of the transgenic and non-transgenic maize plants decreased gradually with the time of 2,4-D treatment, but the decrease of the latter was significantly higher than that of the former. The H _ 2O _ 2 accumulation of wild-type plant leaves treated with 2,4-D was significantly higher than that of the transgenic plants, and the content of malondialdehyde (MDA) in the former was significantly higher than that of the latter, 3.34 and 2.08. m The activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in transgenic plants was 34.88, 4.10 and 31.56U 路 mg-1 (protein) after 3 days of 2,4-D treatment, which was 1.65, 1.4 and 1.7 times (significant difference) of wild type. The results showed that the tfd A gene maize significantly increased the resistance to the herbicide 2,4-D.
【作者单位】：贵州大学农业生物工程研究院/生命科学学院山地植物资源保护与种质创新省部共建教育部重点实验室;贵州省农业科学院;
【基金】：国家转基因生物新品种培育科技重大专项(2016ZX080-10003-009) 贵州省科技厅转基因专项(黔科合2004NGZ004)~~
【分类号】：S482.4;S513

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