水稻低温应答基因OsLCL3的分离与功能鉴定

发布时间：2019-07-05 21:14

【摘要】：为探明水稻响应低温的分子机制,改良水稻对低温的抗性,从水稻低温诱导表达谱中挑选了1个受低温诱导的基因OsLCL3,通过实时定量PCR和启动子驱动GUS报告基因转化水稻的方法验证其功能。对OsLCL3进行同源蛋白搜索,发现它属于DUF761超家族,并对这个超家族中的部分蛋白序列进行了系统树分析;通过水稻原生质体瞬时表达系统,初步确定OsLCL3蛋白定位于细胞核和细胞质中。在水稻中超量表达OsLCL3基因,并对转基因植株进行低温胁迫处理,发现超量表达OsLCL3的植株在低温胁迫下的电导率显著低于野生型对照植株,胁迫后超量表达植株的存活率显著高于野生型对照植株。结果表明,OsLCL3可能在水稻对低温的应答中起着重要作用。
[Abstract]:In order to find out the molecular mechanism of rice response to low temperature and improve the resistance of rice to low temperature, a low temperature induced gene OsLCL3, was selected from the low temperature induction expression profile of rice to verify its function by real time quantitative PCR and promoter driven GUS reporter gene transformation into rice. The homologous protein search of OsLCL3 was carried out, and it was found that it belonged to DUF761 superfamily, and some protein sequences of DUF761 superfamily were analyzed by systematic tree analysis, and the localization of OsLCL3 protein in nucleus and cytoplasm was preliminarily determined by transient expression system of rice protoplasts. The OsLCL3 gene was overexpressed in rice and the transgenic plants were treated with low temperature stress. It was found that the electrical conductivity of the plants overexpressing OsLCL3 under low temperature stress was significantly lower than that of the wild type control plants, and the survival rate of the plants overexpressed after stress was significantly higher than that of the wild type control plants. The results showed that OsLCL3 may play an important role in the response of rice to low temperature.
【作者单位】： 华中农业大学作物遗传改良国家重点实验室;
【基金】：转基因生物新品种培育重大专项(2013ZX08009-003-002)
【分类号】：S511
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本文编号：2510814