日本结缕草‘胶东青’DREB2.2基因克隆及表达模式研究

发布时间：2019-07-06 09:02

【摘要】：DREB(dehydration responsive element binding protein)是植物中普遍存在的一类重要转录因子,参与植物逆境响应和生长发育过程。以日本结缕草‘胶东青’为材料,克隆获得了DREB2.2基因的编码区序列,分析了该基因的生物信息学特征,通过半定量PCR技术检测其逆境表达模式。测序结果表明,‘胶东青’DREB2.2基因存在长、短两个转录本。长转录本DREB2.2-L编码区长1 067 bp,多含一个长50 bp的序列,阅读框提前终止,仅推测编码65个氨基酸。短转录本DREB2.2-S编码区长1 017 bp,推测编码338个氨基酸,蛋白质分子量37.3 KD,等电点p I为4.86,含有一个保守的AP2结构域和核定位序列,属于DREB亚家族A-2组成员。半定量PCR结果显示,DREB2.2-S和DREB2.2-L在正常生长条件下有表达,低温胁迫时表达量上调,胁迫2 h时最高,干旱胁迫2 h和24 h时轻度上调表达,高盐胁迫下表达量无显著变化。在相同条件下,DREB2.2-L的表达量均略高于DREB2.2-S。
[Abstract]:DREB (dehydration responsive element binding protein) is an important transcription factor in plants, which is involved in plant stress response and growth and development. The coding region sequence of DREB2.2 gene was cloned from Zoysia japonica 'Jiaodong Qing'. The bioinformatics characteristics of the gene were analyzed and its stress expression pattern was detected by semi-quantitative PCR. The sequencing results showed that there were two transcripts of 'Jiaodong Qing' DREB2.2 gene. The length of the DREB2.2-L coding region of the long transcript is 1,067 bp, which contains a sequence of 50 bp in length. The reading box terminates ahead of time and only speculates that it encodes 65 amino acids. The length of the DREB2.2-S coding region of the short transcript is 1,017 bp, which encodes 338amino acids, and the molecular weight of the protein is 37.3 KD, isoelectric point p I is 4.86. it contains a conserved AP2 domain and nuclear localization sequence, which belongs to group A of the DREB subfamily. The results of semi-quantitative PCR showed that the expression of DREB2.2-S and DREB2.2-L was up-regulated under normal growth conditions, the highest at 2 h under low temperature stress, and slightly up-regulated at 2 h and 24 h under drought stress, but there was no significant change under high salt stress. Under the same conditions, the expression of DREB2.2-L was slightly higher than that of DREB2.2-S..
【作者单位】：中央民族大学生命与环境学院;
【基金】：国家自然科学基金项目(31100507) 中央民族大学大学生创新训练项目(BEIJ2014110012;URTP2014110029;GCCX-2015110020)
【分类号】：S688.4

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