陆地棉种系品质性状全基因组关联分析及基因鉴定

发布时间：2021-10-17 11:49

　　陆地棉在全世界棉花生产中占95%,是一种具有天然可纺织纤维的作物,也是一种油料作物。作为一种相比传统遗传图谱更好的工具,全基因组关联分析（GWAS）被广泛应用于研究复杂性状的遗传变异。本研究中,我们利用80K Illumina Infinium检测半野生陆地棉自然群体高密度的SNPs,采用GWAS技术对5个纤维质量性状:纤维伸长（FE）,纤维长度（FL）,纤维马克隆值（FM）,纤维强度（FS）和纤维整齐度（FU）进行分析。对在两地三年种植的半野生陆地棉材料的纤维性状进行评价,发现半野生陆地棉材料的纤维质量表现出较高的多样性。FE值为2-8%平均为6.06%,CV%值为17.32、FL值为19.61-32.8mm,平均为24.48mm,CV%值为8.63、FM值为2.1-6.5,平均为4.33,CV%值为16.65、FS值为18.23-34.00 cN/tex,平均为24.24 cN/tex,CV%值为8.17、FU值为74.1-87.7,平均为81.35,CV%值为2.63。根据相关性分析,FL和FU,FL和FS,FU和FS,FM和FE,FE和FS呈正相关,FL和FM,FL和FE,FU... 

【文章来源】：中国农业科学院北京市

【文章页数】：105 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 Uses and production of cotton
    1.2 Geographical distribution and morphological characteristics
    1.3 Cotton fibers development
    1.4 Molecular markers in cotton
        1.4.1 Restriction fragment length polymorphism (RFLP)
        1.4.2 Random amplified polymorphic DNA (RAPD)
        1.4.3 Amplified fragment length polymorphism (AFLP)
        1.4.4 Simple sequence repeats (SSR)
        1.4.5 Single nucleotide polymorphism (SNP)
    1.5 Method of QTL mapping
        1.5.1 Linkage mapping
        1.5.2 Linkage mapping in cotton
        1.5.3 Association mapping
            1.5.3.1 Types of association mapping
                1.5.3.1.1 Candidate gene based association mapping
                1.5.3.1.2 Genome-wide association mapping
    1.6 Association mapping in cotton
        1.6.1 SSR based association studies
            1.6.1.1 Fiber quality
            1.6.1.2 Yield and yield components
            1.6.1.3 Biotic and abiotic stress
        1.6.2 SNP based association studies
            1.6.2.1 Fiber quality
            1.6.2.2 Yield and yield components
            1.6.2.3 Cotton seed nutrition
            1.6.2.4 Salt stress
            1.6.2.5 Early Maturity
    1.7 Population structure
    1.8 Linkage disequilibrium (LD)
    OBJECTIVES
CHAPTER 2 MATERIAL AND METHODS
    2.1 Plant materials
    2.2 Field experiment and phenotyping
    2.3 DNA extraction and SNP genotyping
    2.4 Population structure
    2.5 Linkage disequilibrium
    2.6 Genome-wide association study
    2.7 Candidate genes and gene expression
CHAPTER 3 RESULTS
    3.1 Fiber quality analysis
    3.2 SNPs density
    3.3 Population structure
    3.4 Linkage disequilibrium
    3.5 Marker-trait association
        3.5.1 Fiber elongation
        3.5.2 Fiber length
        3.5.3 Fiber micronaire
        3.5.4 Fiber Strength
        3.5.5 Fiber uniformity
    3.6 Potential candidate genes and genes expressions
CHAPTER 4 DISCUSSION
    4.1 Phenotypic performance
    4.2 Population structure
    4.3 Linkage disequilibrium
    4.4 Marker–trait association
    4.5 Candidate genes and genes expression
CHAPTER 5 CONCLUSION
REFERENCES
APPENDIX
ACKNOWLEDGEMENT
RESUME


【参考文献】：
期刊论文
[1]中国3大主产棉区棉花品种遗传多样性的RAPD及其与农艺性状关系的研究[J]. 别墅,孔繁玲,周有耀,张光梅,张群远,王孝纲.  中国农业科学. 2001(06)



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