Development of oligonucleotide probes for FISH karyotyping i

发布时间：2021-03-04 21:13

　　Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement. Owing to the limited number of probes available for fluorescence in situ hybridization（FISH）, the resolution at which the karyotype of H. villosa can be characterized is poor, hampering accurate characterization of small segmental alien introgressions. We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H. villosa chromosome 6 V（6 VS）. FISH with... 

【文章来源】：The Crop Journal. 2020,8(04)

【文章页数】：6 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant material
    2.2. DNA sequence of H.villosa chromosome 6VS and oligonucleotide probe design
    2.3. Cell cycle synchronization and preparation of mitotic chromosomes
    2.4. FISH and fluorescence microscopy and imaging
3. Results and discussion
Declaration of competing interest



本文编号：3063930