Effects of GS3 and GL3.1 for Grain Size Editing by CRISPR/Ca

发布时间：2021-03-24 22:14

　　Grain size is one of key agronomic traits associated with grain yield and grain quality. Both major quantitative trait loci GS3 and GL3.1 play a predominant role in negative regulation of grain size. In this study, a CRISPR/Cas9-mediated multiplex genome editing system was used to simultaneously edit GS3 and GL3.1 in a typical japonica rice Nipponbare. In T₁ generation, we found that gs3 formed slender grain with lower chalkiness percentage, while gs3gl3.1 produced larger grain with h... 

【文章来源】：Rice Science. 2020,27(05)SCICSCD

【文章页数】：10 页

【文章目录】：
MATERIALS AND METHODS
    Plant materials and growth conditions
    Vector construction and transformation
    Mutation detection in T0 transgenic plants
    Measurement of rice agronomic traits
RESULTS
    Mutations of GS3 and GL3.1 using CRISPR/Cas9system
    Characterization of gs3 and gs3gl3.1 mutation on grain size
    Impacts of gs3 and gs3gl3.1 mutation on grain appearance quality
    Evaluation of gs3 and gs3gl3.1 mutation on other vital agronomic traits
DISCUSSION
SUPPLEMENTAL DATA


【参考文献】：
期刊论文
[1]Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice[J]. Rongfang Xu,Yachun Yang,Ruiying Qin,Hao Li,Chunhong Qiu,Li Li,Pengcheng Wei,Jianbo Yang.  Journal of Genetics and Genomics. 2016(08)



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