水稻苗期耐盐性状的关联分析研究

发布时间：2021-04-21 20:13

　　盐胁迫是影响作物生长及产量的主要胁迫因素之一。随着全球气候变暖,环境恶化,每年有大量土地盐碱化,严重限制了粮食产量。水稻是世界主要粮食作物,苗期对盐胁迫非常敏感,因此培育耐盐品种是水稻主要育种方向之一。水稻耐盐性是由大量QTL/基因控制的复杂数量性状,遗传机制复杂,利用遗传基础丰富的种质资源及全基因组关联分析技术,有助于高效挖掘其耐盐性的遗传学基础。本研究在3000份水稻种质资源测序项目基础上,选取来自不同国家的426份种质资源（62%籼稻资源,28%粳稻资源,1%Aus稻,1%香稻以及8%Admixture）为研究材料,结合2,820,594个SNP（single nucleotide polymorphism）标记（籼稻2,094,607个,粳稻1,449,167个）用于QTL/基因定位。研究调查分析了426份水稻种质资源在盐胁迫及对照条件下11个耐盐相关形态、生理性状特征及盐胁迫下的适应性反应差异。11个性状包括盐处理下水稻苗存活指数（SSI）,苗高/根长（SL/RL）,地上部/根干重（SDW/RDW）,地上部/根部的钠离子含量（SNC/RNC）,地上及根部的钾离子含量（SKC/... 

【文章来源】：中国农业科学院北京市

【文章页数】：95 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
LIST OF ABBREVIATIONS
1 CHAPTER 1 INTRODUCTION
    1.1 BACKGROUND
    1.2 CHALLENGES: GLOBAL RICE PRODUCTION
    1.3 SALINITY EXTENT AND DISTRIBUTION
    1.4 RESPONSE OF PLANT UNDER SALINITY STRESS
    1.5 MECHANISM OF SALT TOLERANCE
    1.6 RICE RESPONSE TO SALINITY STRESS
        1.6.1 Shoot length and root length under salt stress
        1.6.2 Biomass under salt stress
    1.7 IMPLICATION OF MOLECULAR MARKER FOR GENETIC MAPPING
        1.7.1 Single nucleotide polymorphism
        1.7.2 Implementation of SNPs as marker
        1.7.3 Genome-wide association study （GWAS）
        1.7.4 Candidate gene approach
        1.7.5 Linkage disequilibrium and haplotype analysis
    1.8 QUANTITATIVE TRAIT LOCI （QTL）
    1.9 QTLS MAPPING FOR SALINITY TOLERANCE
    1.10 AIMS AND OBJECTIVES
2 CHAPTER 2 MATERIALS AND METHODS
    2.1 PLANT MATERIALS
    2.2 EXPERIMENTAL SITE AND GROWING CONDITION
    2.3 PHENOTYPING OF SEEDLING
        2.3.1 Plant growth measurement
        2.3.2 Seedling survival Index （SSI）
+ and K⁺ concentration in shoot and root">        2.3.3 Measurement of Na⁺ and K⁺ concentration in shoot and root
        2.3.4 Measurement of salinity response index （SRI）
    2.4 GENOTYPING AND SNP IDENTIFICATION
    2.5 STATISTICAL ANALYSIS
        2.5.1 Phenotypic data analysis
        2.5.2 Population genetic analysis
3 Chapter 3 RESULTS
    3.1 IDENTIFICATION OF ELITE GERMPLASMS THROUGH PHYSIOLOGICALCHARACTERIZATION AT SEEDLING STAGES
        3.1.1 Seedling survival index （SSI）
        3.1.2 Shoot length （cm）
        3.1.3 Root length （cm）
        3.1.4 Shoot dry weight （mg）
        3.1.5 Root dry weight （mg）
+ concentration (mg ml^-1) in shoot">        3.1.6 Na⁺ concentration (mg ml^-1) in shoot
+ concentration (mg ml^-1) in shoot">        3.1.7 K⁺ concentration (mg ml^-1) in shoot
+ concentration (mg ml^-1) in root">        3.1.8 Na⁺ concentration (mg ml^-1) in root
+ concentration (mg ml^-1) in root">        3.1.9 K⁺ concentration (mg ml^-1) in root
+/K⁺ ratio in shoot">        3.1.10 Na⁺/K⁺ ratio in shoot
+/K⁺ ratio in root">        3.1.11 Na⁺/K⁺ ratio in root
        3.1.12 Correlation analysis among traits in germplasm
        3.1.13 Performance of selected germplasm at salinity response index
    3.2 IDENTIFICATION OF QTLS ASSOCIATED WITH SALINITY TOLERANCE AT THE SEEDLINGSTAGE THROUGH GWAS
        3.2.1 QTLs for seedling survival index （SSI）
        3.2.2 QTLs for shoot length
        3.2.3 QTLs for root length
        3.2.4 QTLs for shoot dry weight
        3.2.5 QTLs for root dry weight
+ concentration of shoot">        3.2.6 QTLs for Na⁺ concentration of shoot
+ concentration of root">        3.2.7 QTLs for Na⁺ concentration of root
+ concentration of root">        3.2.8 QTLs for K⁺ concentration of root
+/ K⁺ concentration of shoot">        3.2.9 QTLs for Na⁺/ K⁺ concentration of shoot
        3.2.10 Identified QTL regions of QTLs in different traits
    3.3 IDENTIFICATION OF CANDIDATE GENES
        3.3.1 Candidate genes for QTL q SSI-7.1 and q SSI-7.2
        3.3.2 Candidate genes for q SLR-9
        3.3.3 Candidate genes for q RNCR-9.2 and q RNCR-11.1
        3.3.4 Candidate genes for q RKCR-3 and q RKCR-10.1
        3.3.5 Candidate genes for q SNKR-10.2
4 Chapter 4 DISCUSSION
    4.1 PHYSIOLOGICAL CHARACTERIZATION AT SEEDLING STAGE
    4.2 IDENTIFICATION OF QTL USING GWAS
    4.3 CANDIDATE GENE IDENTIFICATION USING HAPLOTYPE ANALYSIS
5 Chapter 5 SUMMARY AND CONCLUSIONS
References
APPENDICES
Acknowledgement
Resume


【参考文献】：
期刊论文
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