盐胁迫下棉花幼苗（Gossypium hirsutum L.）对外源甜菜碱和水杨酸的生理生化特性响应

发布时间：2021-04-23 16:55

　　土壤盐分的升高（尤其是NaCl所引起的）是极为严重的非生物胁迫,抑制了全球约20%灌溉土地的植物生产力。棉花是当今世界上公认的规模最大的纺织纤维,也是重要的耐盐作物,已成为盐碱地主要的栽培作物。甜菜碱（Glycine betaine,GB）是一种具有活跃渗透能力的化合物,在植物抵御高寒和高盐等非生物胁迫过程发挥着重要作用,水杨酸（Salicylic acid,SA）是一种在调节植物多种生理和生化特性中占有重要地位的植物激素,可以影响植物应对非生物胁迫响应过程。深入研究盐分胁迫条件下喷施甜菜碱（GB）及水杨酸（SA）对棉花植株生理、生化特性的影响过程,探索喷施外源物质对棉花适应盐分胁迫的调控机理,对于补充完善施用外源物质（如GB和SA）对棉花耐盐能力调控理论具有重要的理论与实践意义。本研究旨在探讨叶面喷施甜菜碱（GB）和水杨酸（SA）对高盐（NaCl）胁迫条件下棉花幼苗生理生化特性的影响。在150 mM NaCl条件下,喷施外源GB设置3个处理（GB浓度分别为2.5、5.0和7.5 mM）,喷施外源SA设置3个处理（SA浓度分别为1.0、1.5和2.0 mM）,不喷施外源物质的处理1个,... 

【文章来源】：中国农业科学院北京市

【文章页数】：74 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Abbreviation
Chapter1 Background
    1.1 Introduction
    1.2 Soil salinization and its impact on plants
        1.2.1 Effects of salinity stress on plant growth
        1.2.2 Effects of salinity stress on plant photosynthesis
        1.2.3 Responses of antioxidant enzymes activities to salt stress
        1.2.4 Effects of salinity stress on nutrient balance
        1.2.5 Effects of salinity stress on hydraulic conductivity
        1.2.6 Mechanism of salt tolerance
        1.2.7 Amelioration of Salinity Stress
    1.3 Glycine betaine
        1.3.1 Natural production and accumulation of GB
        1.3.2 Effects of Glycine Betaine（GB）
        1.3.3 GB and Salt tolerance in plants
        1.3.4 Exogenous GB applications under salt stress
    1.4 Salicylic acid
        1.4.1 Natural production and accumulation of SA
        1.4.2 Exogenous SA applications under salt stress
    1.5 Research hypothesis
    1.6 Research Objectives
    1.7 Technical route
Chapter2 Research methodology
    2.1 Study site and experimental design
    2.2 Measurements
        2.2.1 Leaf gas exchange and chlorophyll fluorescence
        2.2.2 The key photosynthetic enzymes and photosynthetic pigments
        2.2.3 Antioxidant enzymes,MDA and LRWC
        2.2.4 Experimental Soil EC and p H
        2.2.5 Plant growth characteristics
        2.2.6 Endogenous GB,SA,nitrogen and ions contents
    2.3 Statistical analysis
Chapter3 Responses of leaf gas exchange attributes,photosynthetic pigments and antioxidant enzymes in Na Cl-stressed cotton（Gossypium hirsutum L.）seedlings to exogenous GB and SA
    3.1 Introduction
    3.2 Results
        3.2.1 Responses of endogenous GB and SA to exogenous GB and SA
        3.2.2 Response of leaf gas exchange to exogenous GB and SA
        3.2.3 Response of photosynthetic enzymes MDA and LRWC to exogenous GB and SA
        3.2.4 Response of chlorophyll fluorescence to exogenous GB and SA
        3.2.5 Responses of photosynthetic pigments to exogenous GB and SA
        3.2.6 Responses of antioxidant enzymes to exogenous GB and SA
        3.2.7 Relationships between Pn,FV/FM and chlorophyll a and chlorophyll b content
    3.3 Discussion
    3.4 Summary
Chapter4 Application of exogenous protectants mitigates salt-induced Na+toxicity and sustains cotton（Gossypium hirsutum L.）seedlings growth:comparison of GB and SA
    4.1 Introduction
    4.2 Results
        4.2.1 Influence of exogenous GB and SA on growth and nitrogen accumulation
        4.2.2 Influence of exogenous GB and SA on PLC
        4.2.3 Effect of exogenous GB and SA on ion contents in the shoot and roots of seedlings
        4.2.4 Relationship between PLC and shoot nutrients
    4.3 Discussion
    4.4 Summary
Chapter5 Conclusions and Recommendations
    5.1 Conclusions
    5.2 Recommendations
References
Acknowledgements
Author’s Biography


【参考文献】：
期刊论文
[1]根施甜菜碱对水分胁迫下烟草幼苗光合机构的保护[J]. 马新蕾,王玉军,谢胜利,李峰,王玮.  植物生理与分子生物学学报. 2006(04)
[2]硅改善盐胁迫下库拉索芦荟生长和离子吸收与分布(英文)[J]. 徐呈祥,刘友良,郑青松,刘兆普.  植物生理与分子生物学学报. 2006(01)
[3]水杨酸和热锻炼诱导的高羊茅幼苗的耐热性与抗氧化的关系[J]. 何亚丽,刘友良,陈权,卞爱华.  植物生理与分子生物学学报. 2002(02)



本文编号：3155692