陆地棉纤维品质性状QTL鉴定与qFS21精细定位

发布时间：2021-04-29 19:49

　　棉花是世界上最重要的天然纤维作物,也是重要植物油和蛋白来源作物。此外,棉花相关产业为全世界提供了大量就业机会。因此,棉花在世界经济发展中发挥着重要的作用。棉花作为纤维作物,其产量多少是棉农主要关心的问题,然而纺织业关心的是棉花纤维品质。棉花品质性状主要包括纤维长度、比强度和细度等,这些性状是复杂的数量遗传,其表现容易受到环境因素的影响。由于棉花产量与品质间存在不利相关关系,因此传统的育种方法改良棉花产量与纤维品质相对较难。在定位数量性状基因座（quantitative trait locus,QTL）的基础上,利用与目标性状QTL紧密连锁的分子标记进行分子标记辅助选择（Marker assisted selection,MAS）是一种有效的数量性状改良方法。随着棉花基因组测序的完成,QTL鉴定的效率得到了显著的提高。近年来出现了许多以第二代测序技术为依托的群体基因型检测技术已广泛应用于,如基于测序的基因分型（GBS）、限制性相关DNA测序（Restriction-site associated DNA sequencing,RAD-seq）、特定位点扩增片段测序（Specific-lo... 

【文章来源】：西南大学重庆市 211工程院校 教育部直属院校

【文章页数】：108 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
Chapter one Introduction
    1.1 Classification of Gossypium
    1.2 Cotton fiber development
        1.2.1 Fiber initiation
        1.2.2 Fiber elongation
        1.2.3 Fiber secondary wall thickening
        1.2.4 Fiber dehydration and maturity
    1.3 Genes controlling the cotton fiber development
        1.3.1 Fiber initiation related genes
        1.3.2 Fiber elongation related genes
        1.3.3 Fiber secondary wall thickening related genes
        1.3.4 Fiber dehydration and maturation related genes
    1.4 Cotton genomics
    1.5 Recent advances in cotton genetic map construction
        1.5.1 Interspecific genetic map of cotton
        1.5.2 Intraspecific genetic map of Upland Cotton
    1.6 Progress in mapping of important agronomic traits in cotton
        1.6.1 Preliminary QTL mapping for cotton fiber quality
        1.6.2 Advances in fine-mapping of important agronomic traits of cotton
Chapter Two Whole Genome QTL detection for fibre quality traits
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Mapping Population
        2.2.2 Extraction and purification of total genomic DNA
        2.2.3 Genotyping of SSR primers
        2.2.4 SLAF library construction and high-throughput sequencing
        2.2.5 Sequencing data grouping and genotyping
        2.2.6 Linkage map construction
        2.2.7 Phenotypic data analysis
        2.2.8 Preliminary QTL detection for fiber quality
    2.3 Results and analysis
        2.3.1 Phenotypic analysis of fiber quality traits
        2.3.2 Analysis of SLAF-seq data and SLAF markers
        2.3.3 Construction of the genetic map
        2.3.4 Segregation distortion
        2.3.5 Collinearity between the genetic and the physical map
        2.3.6 QTL mapping
    2.4 Discussion
        2.4.1 Prominent features of SLAF-seq
        2.4.2 SLAF genetic map
        2.4.3 Segregation Distortion
        2.4.4 QTL cluster
        2.4.5 Stable and common QTL
    2.5 Conclusion
Chapter Three fine-mapping qFS21
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Plant Material
        3.2.2 Total genomic DNA extraction from genome
        3.2.3 Genotyping
        3.2.4 Construction of genetic map and QTL mapping
        3.2.5 RNA Extraction
        3.2.6 Parental expression profiling
    3.3 Results and analysis
        3.3.1 Fiber quality analysis
        3.3.2 Construction of high density genetic map in F2 Population
        3.3.3 QTL Mapping for fiber quality traits in F2 population
        3.3.4 Fine-mapping of Fiber quality QTL in F2:3 population
        3.3.5 Identification of candidate genes
    3.4 Discussion
        3.4.1 Fine-mapping population
        3.4.2 Pleiotropism or linkage for QTL affecting multiple fiber traits
        3.4.3 Candidate gene identification strategy
    3.5 Conclusion
        3.5.1 Construction of high density genetic map
        3.5.2 QTL fine-mapping
        3.5.3 Identification of candidate genes
References
Appendix
Acknowledgement
Publications


【参考文献】：
期刊论文
[1]陆地棉优质纤维QTL的分子标记筛选及优质来源分析[J]. 胡文静,张晓阳,张天真,郭旺珍.  作物学报. 2008(04)



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