A bHLH transcription activator regulates defense signaling b

发布时间：2021-05-21 07:53

　　Crosstalk between plant hormone signaling pathways is vital for controlling the immune response during pathogen invasion. Salicylic acid（SA） and jasmonic acid（JA） often play important but antagonistic roles in the immune responses of higher plants. Here,we identify a basic helix-loop-helix transcription activator, OsbHLH6, which confers disease resistance in rice by regulating SA and JA signaling via nucleo-cytosolic trafficking in rice（Oryza sativa）. OsbHLH6 expression was upregulated during Ma... 

【文章来源】：Journal of Integrative Plant Biology. 2020,62(10)SCICSCD

【文章页数】：22 页

【文章目录】：
INTRODUCTION
RESULTS
    Osb HLH6 decreases blast disease resistance
    Jasmonic acid activates Osb HLH6 expression
    Osb HLH6 regulates JA and SA signaling
    Cytosolic OsbHLH6 cannot activate JA signaling
    Osb HLH6 interacts with Os MYC2
    Osb HLH6 competes with Os JAZ1 to bind Os MYC2
    Osb HLH6 interacts with Os NPR1 in the cytoplasm
    Osb HLH6 interferes with the association of Os NPR1with r TGA2.1 and suppresses SA signaling
DISCUSSION
MATERIALS AND METHODS
    Plant and fungi growth conditions
    Plant infection assay
    Measurement of JA,JA‐Ile,and SA by HPLC‐MS/MS
    RNA extraction and RT‐q PCR assay
    Library preparation and bioinformatics analysis
    Rice protoplast transformation and Bi FC assay
    GST and MBP pull‐down assays
    Co‐immunoprecipitation assays
    Electrophoresis mobility shift assay
    Osb HLH6 polyclonal antibody preparation
    Transactivation assay in N.benthamiana leaves
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION



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