Increasing fidelity and efficiency by modifying cytidine bas

发布时间：2021-06-01 02:54

　　The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3（BE3） and targeted activation-induced cytidine deaminase（CDA）（target-AID） systems by coexpressing three copies of free uracil–DNA glycosylase（UDG） inhibitor（UGI）. The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated ri... 

【文章来源】：The Crop Journal. 2020,8(03)SCICSCD

【文章页数】：7 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Vector construction
    2.2. Rice transformation
    2.3. DNA extraction and genotyping
3. Results
4. Discussion
Declaration of Competing Interest


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期刊论文
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