外源褪黑素对玉米苗期盐旱胁迫下抗逆生理及灌浆期抗衰老能力与激素应答的影响
发布时间：2021-06-22 20:47
非生物胁迫可导致植物形态、生理和结构上的多种损害,最终导致农作物减产。水分短缺是主要的环境限制之一,会引起作物活性氧（ROS）的过量产生,对细胞膜造成氧化损伤,降解光合色素,降低蛋白质、酶活性及产量。在胁迫条件下,植物生长调节剂（PGRs）对植物生理的调节起至关重要的作用。褪黑激素是一种重要的生物活性激素,特别是在非生物胁迫条件下,对植物生长和发育有重要作用。本文研究设计了盆栽和两年的大田试验,通过盆栽试验研究了干旱和盐胁迫条件下幼苗外源褪黑素喷施和灌根对玉米早期生长、光合特性和保护酶活性的影响;通过大田试验研究了褪黑素浸种和九叶期叶面施用对延缓叶片衰老、抗氧化防御机制、激素响应、籽粒灌浆速率和玉米产量的影响,主要研究结果如下:1. 盆栽试验在玉米出苗两周后开始干旱胁迫（40%-45%FC）后,进行褪黑素不同浓度的叶面喷施（0,25,50,75 and 100 m M）和土壤浇灌处理（MT1-30,MT2-60,and MT3-90μM）,研究了褪黑素对干旱胁迫下玉米幼苗生长、光合特性和抗氧化防御系统的影响。结果表明,褪...
【文章来源】：西北农林科技大学陕西省 211工程院校 985工程院校 教育部直属院校
【文章页数】：121 页
【学位级别】：博士
【文章目录】：
ABSTRACT
摘要
CHAPTER1 REVIEW OF LITERATURE
1.1 Research background
1.2 Plant growth regulators(PGRs)melatonin and its role in plants
1.3 Action of melatonin in plant
1.3.1 Effect of melatonin on indole-3-acetic acid(IAA)in plants
1.3.2 Effect of melatonin on gibberellin(GAs)in plants
1.3.3 Effect of melatonin on Cytokinin(CK)in plants
1.3.4 Effect of melatonin on abscisic acid(ABA)in plants
1.4 Effects of melatonin attribute on plant growth under drought stress condition
1.4.1 Drought stress and its effect on plant growth
1.4.2 Role of melatonin on plant under drought stress
1.5 Application of melatonin on antioxidant defense system,photosynthetic capacity and process of leaf senescence
1.5.1 Effects of chlorophyll pigments degradation on leaf senescence
1.5.2 Role of antioxidant enzyme and reactive oxygen species(ROS)accumulations in maize
1.5.3 Effect of melatonin on antioxidant defense mechanism and leaf senescence process
1.6 Effect of exogenous melatonin on seed filling rate and hormonal cross-talk changes on maize crop
1.6.1 Role of melatonin on seed filling rate and endogenous hormone contents
1.6.2 Effect of plant hormones content in seed filling rate
1.7 Effect of PGRs on photosynthetic efficiency and antioxidant defense system of maize seedling under salt stress condition
1.7.1 Effect of salinity stress on maize seedling
1.7.2 Effect of PGRs on antioxidant defense system of maize seedling under salinity stress condition
1.8 Objectives of the research
1.9 Research contents
1.10 Systematic diagram
CHAPTER2 MATERIALS AND METHODS
2.1 Description of the experimental site
2.2 Plant material and experimental treatments of pots
2.3 Plant material and experimental treatments of field
2.4 Sampling and measurement
2.4.1 Measurement of growth morphology
2.4.2 Measurement of leaf area plant-1(cm2)
2.4.3 Determination of leaf relative water content(RWC)
2.4.4 Measurement of chlorophyll and carotenoids
2.4.5 Photosynthetic gas exchange
2.4.6 Determination of antioxidant enzyme activity
2.4.7 Measurement of soluble protein,proline and soluble sugar contents
2.4.8 Evaluation of malondialdehyde(MDA)and ROS(O2-and H2O2)
2.4.9 Plant sampling measurements for seed filling rate and endogenous hormones content
2.4.10 Total dry matter plant~(-1)
2.4.11 Seed filling process
2.4.12 Measurement of endogenous hormone contents
2.4.13 Ear characteristics and grain yield
2.5 Statistical analysis
CHAPTER3 EXOGENOUS MELATONIN CONFERS DROUGHT STRESS BY PROMOTING PLANT GROWTH,PHOTOSYNTHETIC CAPACITY AND ANTIOXIDANT DEFENSE SYSTEM OF MAIZE SEEDLINGS
3.1 Effect of melatonin on root length and diameter
3.2 Effects of melatonin on the biomass of maize seedlings
3.3 Stem diameter and plant height
3.4 Leaf relative water content
3.5 Chlorophyll and carotenoid content
3.6 Effect of melatonin on leaf gas exchange
3.7 Effects of melatonin on antioxidant enzymes
3.8 Effects of melatonin on H2O2 and malondialdehyde
3.9 Effects of melatonin on soluble protein and proline contents
3.10 Discussion
3.11 Conclusion
CHAPTER4 EXOGENOUS APPLICATION OF MELATONIN INDUCES TOLERANCE TO SALT STRESS BY INCREASING THE PHOTOSYNTHETIC EFFICIENCY AND ANTIOXIDANT DEFENSE SYSTEM OF MAIZE SEEDLING
4.1 Effect of exogenous melatonin on plant growth characteristics and leaf area plant~(-1) under salt stress
4.2 Effect of exogenous melatonin on chlorophyll pigments under salt stress
4.3 Effect of exogenous melatonin on leaf gas exchange under salt stress
4.4 Effect of exogenous melatonin on relative water content(RWC),soluble sugar(SS)and proline contents under salt stress
4.5 Effect of exogenous melatonin on antioxidant enzyme activities under salt stress
4.6 Effect of exogenous melatonin on malondialdehyde(MDA),H_2O_2 content and electrolyte leakage(EL)under salt stress
4.7 Discussion
4.8 Conclusion
CHAPTER5 FOLIAR APPLICATION OF MELATONIN DELAY LEAF SENESCENCE IN MAIZE BY IMPROVING THE ANTIOXIDANT DEFENSE SYSTEM AND ENHANCING PHOTOSYNTHETIC CAPACITY UNDER SEMI-ARID REGIONS
5.1 Melatonin application improved chlorophyll contents and net photosynthetic rate
5.2 Effects of melatonin on MDA,soluble protein and soluble sugar content
5.3 Effect of melatonin on antioxidant enzymes(SOD,POD,and CAT)
5.4 Effects of melatonin on ROS(O_2~-and H_2O_2)accumulation
5.5 Melatonin application increased green leaf area per plant
5.6 Effects of melatonin on grains per ear, hundred-grains weight and grain yield
5.7 Discussion
5.8 Conclusion
CHAPTER6 EXOGENOUS MELATONIN IMPROVED THE SEED FILLING RATE AND ENDOGENOUS HORMONE CONTENTS IN SUMMER MAIZE UNDER SEMI-ARID REGIONS
6.1 Weather condition of the experimental site during2017 and2018 growth seasons of maize in the semi-arid region
6.2 Seed filling process and100 seed weight
6.3 Endogenous hormones during the process of grain filling
6.3.1 IAA content
6.3.2 GA content
6.3.3 Z+ ZR content
6.3.4 ABA content
6.4 Total dry matter plant~(-1)
6.5 Ear characteristics and grain yield
6.6 Correlation between hormones content and characteristics of seed filling
6.7 Discussion
6.8 Conclusion
CHAPTER7 SUMMERY AND MAIN CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
ABOUT THE AUTHOR
LIST OF PUBLISHED AND PEER REVIEWED ARTICLES
【参考文献】：
期刊论文
[1]外源褪黑素对盐胁迫下猕猴桃幼苗酚类物质含量和抗氧化能力的影响[J]. 高帆,夏惠,袁雪侦,黄守义,刘继,梁东. 浙江农业学报. 2017(07)
[2]Physiological Mechanisms of Delaying Leaf Senescence in Maize Treated with Compound Mixtures of DCPTA and CCC[J]. Wang Yong-chao,Gu Wan-rong,Ye Le-fu,Sun Yang,Li Li-jie,Zhang He,Li Jing,Wei Shi. Journal of Northeast Agricultural University（English Edition）. 2015(03)
[3]玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系[J]. 徐云姬,顾道健,张博博,张耗,王志琴,杨建昌. 作物学报. 2013(08)
[4]盐胁迫对2种苹果属植物愈伤组织及组培苗生长和有机渗透调节物质累积的影响[J]. 王锴,张立新,高梅,赵永桂,吕丽霞,张林森,李丙智,韩明玉. 西北农业学报. 2013(02)
[5]Effects of Conservation Tillage on Crop Yield: a Case Study in the Part of Typical Ecological Zones in China[J]. TANG Qiu-xiang1, 2, LI Shao-kun1, XIE Rui-zhi1, ZHANG Jian-xin3, REN Tian-zhi2, LIN Tao4 and GAO Shi-ju1 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 2 Institute of Agricultural Resources and Regionalization, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 3 Xinjiang University of Agriculture, Urumqi 830052, P.R.China 4 Xinjiang Academy of Agricultural Sciences, Urumqi 830043, P.R.China. Agricultural Sciences in China. 2011(06)
[6]水稻弱势粒灌浆机理与调控途径[J]. 杨建昌. 作物学报. 2010(12)
[7]玉米-小麦一年两熟保护性耕作体系试验研究[J]. 刘立晶,高焕文,李洪文. 农业工程学报. 2004(03)
[8]小麦大粒品种内源CA3及ABA含量的变化规律[J]. 高松洁,王文静,夏国军,程水明,刘俊华. 河南农业大学学报. 2000(03)
本文编号：3243512
【文章来源】：西北农林科技大学陕西省 211工程院校 985工程院校 教育部直属院校
【文章页数】：121 页
【学位级别】：博士
【文章目录】：
ABSTRACT
摘要
CHAPTER1 REVIEW OF LITERATURE
1.1 Research background
1.2 Plant growth regulators(PGRs)melatonin and its role in plants
1.3 Action of melatonin in plant
1.3.1 Effect of melatonin on indole-3-acetic acid(IAA)in plants
1.3.2 Effect of melatonin on gibberellin(GAs)in plants
1.3.3 Effect of melatonin on Cytokinin(CK)in plants
1.3.4 Effect of melatonin on abscisic acid(ABA)in plants
1.4 Effects of melatonin attribute on plant growth under drought stress condition
1.4.1 Drought stress and its effect on plant growth
1.4.2 Role of melatonin on plant under drought stress
1.5 Application of melatonin on antioxidant defense system,photosynthetic capacity and process of leaf senescence
1.5.1 Effects of chlorophyll pigments degradation on leaf senescence
1.5.2 Role of antioxidant enzyme and reactive oxygen species(ROS)accumulations in maize
1.5.3 Effect of melatonin on antioxidant defense mechanism and leaf senescence process
1.6 Effect of exogenous melatonin on seed filling rate and hormonal cross-talk changes on maize crop
1.6.1 Role of melatonin on seed filling rate and endogenous hormone contents
1.6.2 Effect of plant hormones content in seed filling rate
1.7 Effect of PGRs on photosynthetic efficiency and antioxidant defense system of maize seedling under salt stress condition
1.7.1 Effect of salinity stress on maize seedling
1.7.2 Effect of PGRs on antioxidant defense system of maize seedling under salinity stress condition
1.8 Objectives of the research
1.9 Research contents
1.10 Systematic diagram
CHAPTER2 MATERIALS AND METHODS
2.1 Description of the experimental site
2.2 Plant material and experimental treatments of pots
2.3 Plant material and experimental treatments of field
2.4 Sampling and measurement
2.4.1 Measurement of growth morphology
2.4.2 Measurement of leaf area plant-1(cm2)
2.4.3 Determination of leaf relative water content(RWC)
2.4.4 Measurement of chlorophyll and carotenoids
2.4.5 Photosynthetic gas exchange
2.4.6 Determination of antioxidant enzyme activity
2.4.7 Measurement of soluble protein,proline and soluble sugar contents
2.4.8 Evaluation of malondialdehyde(MDA)and ROS(O2-and H2O2)
2.4.9 Plant sampling measurements for seed filling rate and endogenous hormones content
2.4.10 Total dry matter plant~(-1)
2.4.11 Seed filling process
2.4.12 Measurement of endogenous hormone contents
2.4.13 Ear characteristics and grain yield
2.5 Statistical analysis
CHAPTER3 EXOGENOUS MELATONIN CONFERS DROUGHT STRESS BY PROMOTING PLANT GROWTH,PHOTOSYNTHETIC CAPACITY AND ANTIOXIDANT DEFENSE SYSTEM OF MAIZE SEEDLINGS
3.1 Effect of melatonin on root length and diameter
3.2 Effects of melatonin on the biomass of maize seedlings
3.3 Stem diameter and plant height
3.4 Leaf relative water content
3.5 Chlorophyll and carotenoid content
3.6 Effect of melatonin on leaf gas exchange
3.7 Effects of melatonin on antioxidant enzymes
3.8 Effects of melatonin on H2O2 and malondialdehyde
3.9 Effects of melatonin on soluble protein and proline contents
3.10 Discussion
3.11 Conclusion
CHAPTER4 EXOGENOUS APPLICATION OF MELATONIN INDUCES TOLERANCE TO SALT STRESS BY INCREASING THE PHOTOSYNTHETIC EFFICIENCY AND ANTIOXIDANT DEFENSE SYSTEM OF MAIZE SEEDLING
4.1 Effect of exogenous melatonin on plant growth characteristics and leaf area plant~(-1) under salt stress
4.2 Effect of exogenous melatonin on chlorophyll pigments under salt stress
4.3 Effect of exogenous melatonin on leaf gas exchange under salt stress
4.4 Effect of exogenous melatonin on relative water content(RWC),soluble sugar(SS)and proline contents under salt stress
4.5 Effect of exogenous melatonin on antioxidant enzyme activities under salt stress
4.6 Effect of exogenous melatonin on malondialdehyde(MDA),H_2O_2 content and electrolyte leakage(EL)under salt stress
4.7 Discussion
4.8 Conclusion
CHAPTER5 FOLIAR APPLICATION OF MELATONIN DELAY LEAF SENESCENCE IN MAIZE BY IMPROVING THE ANTIOXIDANT DEFENSE SYSTEM AND ENHANCING PHOTOSYNTHETIC CAPACITY UNDER SEMI-ARID REGIONS
5.1 Melatonin application improved chlorophyll contents and net photosynthetic rate
5.2 Effects of melatonin on MDA,soluble protein and soluble sugar content
5.3 Effect of melatonin on antioxidant enzymes(SOD,POD,and CAT)
5.4 Effects of melatonin on ROS(O_2~-and H_2O_2)accumulation
5.5 Melatonin application increased green leaf area per plant
5.6 Effects of melatonin on grains per ear, hundred-grains weight and grain yield
5.7 Discussion
5.8 Conclusion
CHAPTER6 EXOGENOUS MELATONIN IMPROVED THE SEED FILLING RATE AND ENDOGENOUS HORMONE CONTENTS IN SUMMER MAIZE UNDER SEMI-ARID REGIONS
6.1 Weather condition of the experimental site during2017 and2018 growth seasons of maize in the semi-arid region
6.2 Seed filling process and100 seed weight
6.3 Endogenous hormones during the process of grain filling
6.3.1 IAA content
6.3.2 GA content
6.3.3 Z+ ZR content
6.3.4 ABA content
6.4 Total dry matter plant~(-1)
6.5 Ear characteristics and grain yield
6.6 Correlation between hormones content and characteristics of seed filling
6.7 Discussion
6.8 Conclusion
CHAPTER7 SUMMERY AND MAIN CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS
ABOUT THE AUTHOR
LIST OF PUBLISHED AND PEER REVIEWED ARTICLES
【参考文献】：
期刊论文
[1]外源褪黑素对盐胁迫下猕猴桃幼苗酚类物质含量和抗氧化能力的影响[J]. 高帆,夏惠,袁雪侦,黄守义,刘继,梁东. 浙江农业学报. 2017(07)
[2]Physiological Mechanisms of Delaying Leaf Senescence in Maize Treated with Compound Mixtures of DCPTA and CCC[J]. Wang Yong-chao,Gu Wan-rong,Ye Le-fu,Sun Yang,Li Li-jie,Zhang He,Li Jing,Wei Shi. Journal of Northeast Agricultural University（English Edition）. 2015(03)
[3]玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系[J]. 徐云姬,顾道健,张博博,张耗,王志琴,杨建昌. 作物学报. 2013(08)
[4]盐胁迫对2种苹果属植物愈伤组织及组培苗生长和有机渗透调节物质累积的影响[J]. 王锴,张立新,高梅,赵永桂,吕丽霞,张林森,李丙智,韩明玉. 西北农业学报. 2013(02)
[5]Effects of Conservation Tillage on Crop Yield: a Case Study in the Part of Typical Ecological Zones in China[J]. TANG Qiu-xiang1, 2, LI Shao-kun1, XIE Rui-zhi1, ZHANG Jian-xin3, REN Tian-zhi2, LIN Tao4 and GAO Shi-ju1 1 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 2 Institute of Agricultural Resources and Regionalization, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 3 Xinjiang University of Agriculture, Urumqi 830052, P.R.China 4 Xinjiang Academy of Agricultural Sciences, Urumqi 830043, P.R.China. Agricultural Sciences in China. 2011(06)
[6]水稻弱势粒灌浆机理与调控途径[J]. 杨建昌. 作物学报. 2010(12)
[7]玉米-小麦一年两熟保护性耕作体系试验研究[J]. 刘立晶,高焕文,李洪文. 农业工程学报. 2004(03)
[8]小麦大粒品种内源CA3及ABA含量的变化规律[J]. 高松洁,王文静,夏国军,程水明,刘俊华. 河南农业大学学报. 2000(03)
本文编号：3243512
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